Isolating and Culturing Mouse Podocyte Cells to Study Diabetic Nephropathy

Elisavet Vasilopoulou

doi:10.1007/978-1-4939-9841-8_5

Isolating and Culturing Mouse Podocyte Cells to Study Diabetic Nephropathy

Methods Mol Biol. 2020:2067:53-59. doi: 10.1007/978-1-4939-9841-8_5.

Author

Elisavet Vasilopoulou^{1

2}

Affiliations

¹ Medway School of Pharmacy, University of Kent, Chatham, Kent, UK. e.vasilopoulou@kent.ac.uk.
² Developmental Biology and Cancer, UCL GOS Institute of Child Health, London, UK. e.vasilopoulou@kent.ac.uk.

PMID: 31701445
DOI: 10.1007/978-1-4939-9841-8_5

Abstract

Diabetic nephropathy is associated with injury and loss of podocytes, specialized epithelial cells that are critical for glomerular filtration. This chapter describes a method of isolating and culturing podocyte cells from mouse adult kidneys. In this way, podocytes with genetic modifications can be obtained from transgenic animals and they can be used to study the effects of the diabetic environment in vitro.

Keywords: Diabetes; Diabetic nephropathy; Glomerulus; Kidney; Podocyte.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cells, Cultured
Diabetic Nephropathies / genetics
Diabetic Nephropathies / pathology*
Disease Models, Animal
Humans
Immunomagnetic Separation
Mice
Mice, Transgenic
Podocytes / pathology*
Primary Cell Culture / methods*
Signal Transduction