Manipulating osa-MIR156f Expression by D18 Promoter to Regulate Plant Architecture and Yield Traits both in Seasonal and Ratooning Rice

Qing Liu; Yi Su; Yunhua Zhu; Keqin Peng; Bin Hong; Ruozhong Wang; Mahmoud Gaballah; Langtao Xiao

doi:10.1186/s12575-019-0110-4

Manipulating osa-MIR156f Expression by D18 Promoter to Regulate Plant Architecture and Yield Traits both in Seasonal and Ratooning Rice

Biol Proced Online. 2019 Nov 4:21:21. doi: 10.1186/s12575-019-0110-4. eCollection 2019.

Authors

Qing Liu^#¹, Yi Su^#^{1

2}, Yunhua Zhu³, Keqin Peng¹, Bin Hong¹, Ruozhong Wang^{1

2}, Mahmoud Gaballah⁴, Langtao Xiao^{1

2}

Affiliations

¹ 1Hunan Provincial Key Laboratory of Phytohormones and Growth Development, Hunan Agricultural University, Changsha, 410128 China.
² 2Southern Regional Collaborative Innovation Center for Grain and Oil Crops in China, Hunan Agricultural University, Changsha, 410128 China.
³ 3Hengyang Medical College, University of South China, Hengyang, 421001 China.
⁴ 4Rice Research and Training Center, Field Crops Research Institute, Agriculture Research Center, Giza, 33717 Egypt.

^# Contributed equally.

Abstract

Background: Rice (Oryza sativa L.) feeds more than half of the world's population. Ratooning rice is an economical alternative to the second seasonal rice, thus increasing the yield of ratooning rice is highly important.

Results: Here we report an applicable transgenic line constructed through the manipulation of osa-MIR156f expression in rice shoot using the OsGA3ox2 (D18) promoter. In seasonal rice, the D18-11 transgenic line showed moderate height and more effective tillers with normal panicle. In ratooning rice, axillary buds outgrew from the basal node of the D18-11 transgenic line before the harvest of seasonal rice. More effective tillers produced by the outgrowth of axillary buds contributed to the plant architecture improvement and yield increase. Additionally, it was found that osa-miR156f down-regulated the expression of tillering regulators, such as TEOSINTE BRANCHED1 (TB1) and LAX PANICLE 1 (LAX1). The expression of DWARF10, DWARF27 and DWARF53, three genes being involved in the biosynthesis and signaling of strigolactone (SL), decreased in the stem of the D18-11 transgenic line.

Conclusion: Our results indicated that the manipulation of osa-MIR156f expression may have application significance in rice genetic breeding. This study developed a novel strategy to regulate plant architecture and grain yield potential both in the seasonal and ratooning rice.

Keywords: Grain yield; Osa-MIR156f; Plant architecture; Ratooning rice; Seasonal rice.