In the present study, lipase production from mutated strain of Fusarium incarnatum KU377454 was optimized through central composite design (CCD) based response surface methodology (RSM). The maximum lipase production (4.01 IU/mL) was obtained within 4 days of incubation using 0.1% CaCl2 concentration and 8% wheat bran concentration. Further, salting out technique was applied for partial purification of lipase. The partially purified lipase was immobilized using Au@Ag bimetallic nanoshell. The characterization of immobilized lipase was carried out by transmission electron microscopy (TEM), field emission scanning electron microscopy (FE-SEM), Fourier transformed infrared (FTIR), energy dispersive X-ray (EDX), X-ray diffraction (XRD) and thermo gravimetric analysis (TGA). The immobilized lipase could retain its 95% of activity after 15 days of storage at 4 °C. Subsequently, Au@Ag immobilized lipase was used for the degradation of waste cooking oil (WCO), which showed higher WCO degradation (85%) compared to the free lipase mediated waste cooking oil degradation (71%). The immobilized lipase could be reused for five times without any loss of its activity.
Keywords: Au–Ag core shells; Characterization; Immobilization; Lipase; Optimization; WCO degradation.
© King Abdulaziz City for Science and Technology 2019.