Nanocomposite packaging regulates extracellular ATP and programed cell death in edible mushroom (Flammulina velutipes)

Food Chem. 2020 Mar 30:309:125702. doi: 10.1016/j.foodchem.2019.125702. Epub 2019 Oct 19.

Abstract

Our previous study indicated that nanocomposite packaging material (Nano-PM) containing nano-Ag, nano-TiO2, nano-SiO2 and nanoattapulgite alleviated postharvest senescence of Flammulina velutipes by regulating respiration and energy metabolism. In this study, extracellular ATP (eATP) and programmed cell death (PCD) were employed as critical factors to further investigate the senescence mechanism of postharvest F. velutipes. Results demonstrated that Nano-PM delayed apyrase activity decrease and stimulated critical oxidative phosphorylation-related gene expression to inhibit eATP content increase, which is a crucial signaling molecule related to delaying senescence. The regulation of eATP resulted in alleviating PCD including chromosomal concentration, DNA fragmentation, Ca2+ influx, high caspase-1 activity and cytochrome c content and leading to high cell viability. Overall, Nano-PM alleviated PCD and postharvest senescence of F. velutipes by regulating extracellular ATP.

Keywords: Extracellular ATP; Flammulina velutipes; Nanocomposite packaging; Programmed cell death.

MeSH terms

  • Adenosine Triphosphate / metabolism*
  • Apoptosis* / drug effects
  • DNA Fragmentation / drug effects
  • Flammulina / drug effects
  • Flammulina / metabolism*
  • Food Packaging / methods*
  • Nanocomposites / chemistry*
  • Nanocomposites / toxicity
  • Nitric Oxide / analysis
  • Silicon Dioxide / chemistry
  • Silver / chemistry
  • Titanium / chemistry

Substances

  • titanium dioxide
  • Nitric Oxide
  • Silver
  • Silicon Dioxide
  • Adenosine Triphosphate
  • Titanium