Kinetic Analysis by Affinity Chromatography

Abstract

Important information on chemical processes in living systems can be obtained by the rates at which these biological interactions occur. This review will discuss several techniques based on traditional and high-performance affinity chromatography that may be used to examine the kinetics of biological reactions. These methods include band-broadening measurements, techniques for peak fitting, split-peak analysis, peak decay studies, and ultrafast affinity extraction. The general principles and theory of each method, as applied to the determination of rate constants, will be discussed. The applications of each approach, along with its advantages and limitations, will also be considered.

Keywords: affinity chromatography; biological interactions; kinetics; peak decay method; peak profiling; plate height method; split-peak method; ultrafast affinity extraction.