The effect of benzo-(1,2,3)-thiadiazole-7-carbothioic acid S-methyl ester (BTH) treatment on regulation of reactive oxygen species metabolism involved in wound healing of potato tubers during postharvest

Hong Jiang; Yi Wang; Changjian Li; Bin Wang; Li Ma; Yingyue Ren; Yang Bi; Yongcai Li; Huali Xue; Dov Prusky

doi:10.1016/j.foodchem.2019.125608

The effect of benzo-(1,2,3)-thiadiazole-7-carbothioic acid S-methyl ester (BTH) treatment on regulation of reactive oxygen species metabolism involved in wound healing of potato tubers during postharvest

Food Chem. 2020 Mar 30:309:125608. doi: 10.1016/j.foodchem.2019.125608. Epub 2019 Oct 16.

Authors

Hong Jiang¹, Yi Wang¹, Changjian Li¹, Bin Wang¹, Li Ma¹, Yingyue Ren¹, Yang Bi², Yongcai Li¹, Huali Xue³, Dov Prusky⁴

Affiliations

¹ College of Food Science and Engineering, Gansu Agricultural University, Lanzhou 730070, China.
² College of Food Science and Engineering, Gansu Agricultural University, Lanzhou 730070, China. Electronic address: biyang@gsau.edu.cn.
³ College of Science, Gansu Agricultural University, Lanzhou 730070, China.
⁴ College of Food Science and Engineering, Gansu Agricultural University, Lanzhou 730070, China; Department of Postharvest Science of Fresh Produce, Agricultural Research Organization, The Volcani Center, Bet Dagan 50250, Israel.

PMID: 31678673
DOI: 10.1016/j.foodchem.2019.125608

Abstract

Benzo-(1,2,3)-thiadiazole-7-carbothioic acid S-methyl ester (BTH) can improve wound healing of potato tubers; however, how the chemical regulates reactive oxygen species (ROS) generation and scavenging during wound healing is not completely understood. BTH at 100 mg·L^-1 regulated changes in ROS generation and scavenging in healing tissues of potato tubers. A higher H₂O₂ content was presented in healing tissues of potato tubers, while cell membrane permeability and malondialdehyde content declined due to BTH treatment. Additionally, the activities and transcript level of enzymes related with ROS generation, including NADPH oxidase, peroxidase and polyamine oxidase, as well as enzymes involved in ROS scavenging, such as superoxide dismutase, catalase, ascorbate peroxidase, and glutathione reductase, were significantly enhanced by BTH treatment. It is suggested that ROS metabolism might play a crucial role in wound healing of potato tubers mediated by BTH during postharvest.

Keywords: Benzo-(1,2,3)-thiadiazole-7-carbothioic acid S-methyl ester (BTH); Potato tuber; Reactive oxygen species (ROS) metabolism; Wound-healing.

MeSH terms

Cell Membrane / drug effects
Cell Membrane Permeability / drug effects
Enzymes / genetics
Enzymes / metabolism
Gene Expression Regulation, Plant / drug effects
Hydrogen Peroxide / metabolism
Malondialdehyde / metabolism
Plant Proteins / genetics
Plant Proteins / metabolism
Plant Tubers / drug effects*
Plant Tubers / metabolism*
Reactive Oxygen Species / metabolism
Solanum tuberosum / drug effects*
Solanum tuberosum / metabolism*
Thiadiazoles / pharmacology*

Substances

Enzymes
Plant Proteins
Reactive Oxygen Species
Thiadiazoles
Malondialdehyde
Hydrogen Peroxide
S-methyl benzo(1,2,3)thiadiazole-7-carbothioate