Influence of hemoglobin and albumin on the NO donation effect of tetranitrosyl iron complex with thiosulfate

Nitric Oxide. 2020 Jan 1:94:69-72. doi: 10.1016/j.niox.2019.10.010. Epub 2019 Oct 31.

Abstract

The effects of deoxyhemoglobin (Hb) and albumin on the NO-donor activity of the anionic tetranitrosyl iron complex with thiosulfate ligands (1) were studied for the first time. It was shown that Hb significantly stabilizes complex 1; in its presence, NO generation from the complex proceeds at a noticeably slower rate. A similar effect is observed when complex 1 is bound to albumin, in which case complex 1 decomposes 27 times slower than in the absence of albumin in the solution. The observed effects provide a prolonged action of complex 1 as NO-donor, which may enhance its potential pharmacological efficacy.

Keywords: Bovine serum albumin; Deoxyhemoglobin; Nitric monoxide; Nitrosyl iron complexes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Albumins / chemistry*
  • Animals
  • Cattle
  • Ferric Compounds / chemistry*
  • Hemoglobins / chemistry*
  • Iron
  • Kinetics
  • Nitrogen Oxides / chemistry*
  • Thiosulfates / chemistry*

Substances

  • Albumins
  • Ferric Compounds
  • Hemoglobins
  • Nitrogen Oxides
  • Thiosulfates
  • dinitrosyl iron complex
  • deoxyhemoglobin
  • Iron