This study investigates the effect of dispensing glucose oxidase enzyme onto the reaction zone of a golden electrode via a jetting dispenser. Each droplet of enzyme solution dispensed onto the reaction zone of golden electrode weighs exactly the same at around 0.4 mg. This study shows that the spring and needle assembly can be controlled by adjusting the stroke value of the stroke adjustment knob to generate different jet dispensing effects, thus affecting the change of droplets of glucose oxidase enzyme solution within the reaction zone of the golden electrode. This study performs experiments using three stroke values, which are 0.8, 1.2, and 1.6 mm. The experimental results show that adjusting the stroke value to change the droplet of the dispensing liquid will significantly affect the accuracy of the test strip reading value. When the stroke value is adjusted to 1.5 mm, the standard deviation of the test strip is 3.8 mg/dL and the coefficient of variation is 4.1%-6.1%. The study suggested that adjusting the stroke value can stabilize the dispensed droplet to increase the stability of test strip reading, improving the accuracy of the test strip reading. This adjustment method can also be applied to the process of other biochemical sensors.
Keywords: Biosensor; glucose oxidase; golden electrode; jetting dispenser; reaction.