Monitoring of the toxic dinoflagellate Alexandrium catenella in Osaka Bay, Japan using a massively parallel sequencing (MPS)-based technique

Satoshi Nagai; Hungyen Chen; Yoko Kawakami; Keigo Yamamoto; Sirje Sildever; Nanako Kanno; Hiroshi Oikawa; Motoshige Yasuike; Yoji Nakamura; Yuki Hongo; Atushi Fujiwara; Takanori Kobayashi; Takashi Gojobori

doi:10.1016/j.hal.2019.101660

Monitoring of the toxic dinoflagellate Alexandrium catenella in Osaka Bay, Japan using a massively parallel sequencing (MPS)-based technique

Harmful Algae. 2019 Nov:89:101660. doi: 10.1016/j.hal.2019.101660. Epub 2019 Sep 12.

Authors

Affiliations

¹ National Research Institute of Fisheries Science, 2-12-4 Fukuura, Kanazawa-ku, Yokohama, Kanagawa, 236-8648, Japan. Electronic address: snagai@affrc.go.jp.
² Department of Agronomy, National Taiwan University, No. 1, Sec. 4, Roosevelt Rd., Taipei, 10617, Taiwan.
³ AXIOHELIX Co. Ltd, -12-17 Kandaizumicho, Chiyoda-ku, Tokyo, 101-0024, Japan.
⁴ Research Institute of Environment, Agriculture and Fisheries, Osaka Prefecture, 2926-1 Tanigawa, Misaki, Sen-Nan, Osaka, 599-0311, Japan.
⁵ National Research Institute of Fisheries Science, 2-12-4 Fukuura, Kanazawa-ku, Yokohama, Kanagawa, 236-8648, Japan.
⁶ Computational Bioscience Research Center, King Abdullah University of Science and Technology, 4700 KAUST, Thuwal, 23955-6900, Saudi Arabia.

PMID: 31672234
DOI: 10.1016/j.hal.2019.101660

Abstract

Since 2002, blooms of Alexandrium catenella sensu Fraga et al. (2015) and paralytic shellfish toxicity events have occurred almost yearly in Osaka Bay, Japan. To better understand the triggers for reoccurring A. catenella blooms in Osaka Bay, phytoplankton community was monitored during the spring seasons of 2012-2015. Monitoring was performed using massively parallel sequencing (MPS)-based technique on amplicon sequences of the 18S rRNA gene. Dense blooms of A. catenella occurred every year except in 2012, however, there was no significant correlation with the environmental parameters investigated. Plankton community diversity decreased before and middle of the A. catenella blooms, suggesting that the decline in diversity could be an indicator for the bloom occurrence. The yearly abundance pattern of A. catenella cells obtained by morphology-based counting coincided with the relative sequence abundances, which supports the effectiveness of MPS-based phytoplankton monitoring.

Keywords: Alexandrium catenella; Massively parallel sequencing; Osaka Bay; Paralytic shellfish poisoning; Plankton assemblages; Species diversity.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bays
Dinoflagellida*
High-Throughput Nucleotide Sequencing
Japan
Phytoplankton