A one-year longitudinal study of enterohemorrhagic Escherichia coli O157 fecal shedding in a beef cattle herd

Abstract

Bovines are the primary reservoir of enterohemorrhagic Escherichia coli (EHEC) O157:H7 and the main source of its transmission to humans. Here, we present a one-year longitudinal study of fecal shedding of E. coli O157. E. coli O157 obtained from recto-anal mucosal samples were characterized by multiplex PCR. The E. coli O157 prevalence ranged from 0.84% in July to 15.25% in November. The confinement within pens resulted in prevalence of 11%. Most animals (61.86%; 75/118) shed E. coli O157 at least in one sampling occasion. Of the positive animals, 82.19%, 16.44%, and 1.37% were stx positive on one, two and three sampling occasions, respectively. All the E. coli O157 isolated strains carried the genes eae and rfb_O157, whereas 11%, 33% and 56% contained stx₁, stx₂ and stx₁/stx₂, respectively. The stx₁/stx₂ and stx₂ types were significantly higher during the grazing and finishing periods, respectively, in comparison with the rearing and grazing periods. The presence of stx_2a subtype was evident in four isolates, whereas stx_2c was present in at least seven. However, both subtypes were present simultaneously in two isolates. The stx₁/stx_2c, stx₁/stx_2d and stx₁/stx_2NT genotypes occurred in 24, 2 and 15 isolates, respectively. The simultaneous occurrence of stx₁ and stx_2c significantly increased during grazing. Some cases of within-pen and between-pen transmission occurred throughout the study. Contagion levels during in-field grazing were higher than during permanent confinement in the pens. Thus, the individual patterns of shedding varied depending on the proportion of animals shedding the bacteria within pens and the time of shedding.

Keywords: Cattle; Escherichia coli O157; Shedding; Virulence genes.