Retinal-based polyene fluorescent probe for selectively detection of Cu2+ in physiological saline and serum

Yang Li; Haichuang Lan; Xia Yan; Xiaotao Shi; Xiao Liu; Shuzhang Xiao

doi:10.1016/j.saa.2019.117565

Retinal-based polyene fluorescent probe for selectively detection of Cu²⁺ in physiological saline and serum

Spectrochim Acta A Mol Biomol Spectrosc. 2020 Feb 15:227:117565. doi: 10.1016/j.saa.2019.117565. Epub 2019 Oct 20.

Authors

Yang Li¹, Haichuang Lan², Xia Yan¹, Xiaotao Shi¹, Xiao Liu³, Shuzhang Xiao⁴

Affiliations

¹ College of Biological and Pharmaceutical Sciences, Engineering Research Center of Eco-environment in Three Gorges Reservoir Region, Ministry of Education, China Three Gorges University, Yichang, Hubei, 443002, PR China.
² College of Biological and Pharmaceutical Sciences, Engineering Research Center of Eco-environment in Three Gorges Reservoir Region, Ministry of Education, China Three Gorges University, Yichang, Hubei, 443002, PR China. Electronic address: haichuanglan@ctgu.edu.cn.
³ Pall Corporation, 25 Harbor Dr, Port Washington, NY, 11050, USA.
⁴ College of Biological and Pharmaceutical Sciences, Engineering Research Center of Eco-environment in Three Gorges Reservoir Region, Ministry of Education, China Three Gorges University, Yichang, Hubei, 443002, PR China. Electronic address: shuzhangxiao@ctgu.edu.cn.

PMID: 31670041
DOI: 10.1016/j.saa.2019.117565

Abstract

Retinal is a flexible natural chromophore and widely present in organisms. The slender conjugated polyene structure retinal is conducive to entering protein structure. In this work, a novel turn-on fluorescent probe for Cu²⁺ based on retinal and phenylenediamine was designed and synthesized. The probe achieved recognition of copper ions in human serum complex protein environment. Furthermore, the high sensitivity, selectivity for Cu²⁺ and the sensing mechanism was also investigated.

Keywords: Chemosensor; Copper cation; Fluorescence; Retinal.

Publication types

Evaluation Study

MeSH terms

Cations, Divalent / analysis
Cations, Divalent / blood
Copper / analysis
Copper / blood*
Fluorescent Dyes / chemistry*
Humans
Limit of Detection
Polyenes / chemistry*
Retinaldehyde / analogs & derivatives*
Saline Solution / analysis
Spectrometry, Fluorescence / methods

Substances

Cations, Divalent
Fluorescent Dyes
Polyenes
Saline Solution
Copper
Retinaldehyde