Dissemination of blaKPC-2 in an NTEKPC by an IncX5 plasmid

Rosineide Cardoso de Souza; Andrei Nicoli Gebieluca Dabul; Camila Maria Dos Santos Boralli; Luíza Zuvanov; Ilana Lopes Baratella da Cunha Camargo

doi:10.1016/j.plasmid.2019.102446

Dissemination of bla_KPC-2 in an NTE_KPC by an IncX5 plasmid

Plasmid. 2019 Nov:106:102446. doi: 10.1016/j.plasmid.2019.102446. Epub 2019 Oct 25.

Authors

Rosineide Cardoso de Souza¹, Andrei Nicoli Gebieluca Dabul², Camila Maria Dos Santos Boralli², Luíza Zuvanov², Ilana Lopes Baratella da Cunha Camargo³

Affiliations

¹ Genetics and Evolution Department, Federal University of São Carlos, São Carlos 13565-905, Brazil.
² São Carlos Institute of Physics, University of São Paulo, São Carlos 13563-120, Brazil.
³ São Carlos Institute of Physics, University of São Paulo, São Carlos 13563-120, Brazil. Electronic address: ilanacamargo@ifsc.usp.br.

PMID: 31669115
DOI: 10.1016/j.plasmid.2019.102446

Abstract

bla_KPC-2 is disseminated worldwide usually in Tn4401, a Tn3-family transposon, and primarily in Klebsiella pneumoniae ST258, a well-known lineage that is distributed worldwide and responsible for several outbreaks. Although occurring rarely, bla_KPC-2 has been described in non-Tn4401 elements (NTE_KPCs), first in China and then in a few other countries. This study reports the dissemination of a bla_KPC-2-carrying NTE_KPC among ST11/CG258 K. pneumoniae strains and ST1642 K. quasipneumoniae subsp. quasipneumoniae AMKP9 in an Amazonian hospital. The dissemination was due to pAMKP10, an ~48 kbp IncX5 plasmid carrying ΔISKpn6/bla_KPC-2/ISKpn27 in a Tn1722-based unit. Although similar to NTE_KPC-Ia from pKP048 described in China, a different transposase is present upstream of ISKpn27. Additionally, mutations were identified downstream of ISKpn27 but did not affect the bla_KPC-2 promoter regions. pAMKP10 conjugated in vitro only from CG258 isolates. Since CG258 strains are generally well adapted to the hospital environment, it is significant that pAMKP10 has found its way into this clinically significant clonal group. The impact of inter- and intraspecies dissemination of NTE_KPCs and IncX5 plasmids harboring carbapenem resistance genes is unknown, but monitoring these plasmids could reveal their dissemination preferences.

Keywords: IncX5 plasmid; K. quasipneumoniae subsp. quasipneumoniae ST1642; Klebsiella pneumoniae; NTE(KPC); Tn1722; bla(KPC-2).

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Anti-Bacterial Agents / pharmacology
DNA Transposable Elements*
Genome, Bacterial
Klebsiella Infections / epidemiology
Klebsiella Infections / microbiology
Klebsiella pneumoniae / classification
Klebsiella pneumoniae / drug effects
Klebsiella pneumoniae / genetics*
Microbial Sensitivity Tests
Molecular Typing
Plasmids / genetics*
Whole Genome Sequencing
beta-Lactamases / genetics*

Substances

Anti-Bacterial Agents
DNA Transposable Elements
beta-Lactamases
beta-lactamase KPC-2, Klebsiella pneumoniae