Industrial fermentation focuses on realizing the uniform of high titer, high yield, and high productivity. Multi-scale analysis and regulation, including molecule level, cell level, and bioreactor level, facilitate global optimization and dynamic balance of fermentation process, which determine high efficiency of biosynthesis, targeted directionality of bioconversion, process robustness, and well-organized system. In this review, we summariz and discuss advances in multi-scale analysis and regulation for fermentation process focusing on the following four aspects: 1) kinetic modeling of metabolic pathways, 2) characteristic of cell metabolism, 3) co-coupling fermentation and purification, and 4) bioreactor design. Integrating multi-scale analysis of fermentation process and integrating multi-scale regulation are expected as an important strategy for realizing highly efficient fermentation by industrial microorganisms.
工业发酵科学致力于实现高产量、高转化率和高生产强度的相对统一。通过从分子、细胞和反应器进行发酵过程多尺度解析与调控，实施全局与动态的优化与控制能够确保发酵过程高效、转化定向、过程稳定和系统有序。本文从发酵微生物代谢途径动力学模型、细胞代谢特性、发酵提取相耦合与反应器设计四个方面，总结和讨论发酵过程多尺度解析与调控的研究进展。整合分析发酵过程不同尺度特征并且针对性地开展多尺度整合调控是实现高效工业微生物发酵的重要策略。.
Keywords: bioreactor design; characteristics of cell metabolism; co-coupling fermentation and purification; kinetic modeling.