Antimicrobial and wound healing properties of a bacterial cellulose based material containing B. subtilis cells

I S Savitskaya; D H Shokatayeva; A S Kistaubayeva; L V Ignatova; I E Digel

doi:10.1016/j.heliyon.2019.e02592

Antimicrobial and wound healing properties of a bacterial cellulose based material containing B. subtilis cells

Heliyon. 2019 Oct 10;5(10):e02592. doi: 10.1016/j.heliyon.2019.e02592. eCollection 2019 Oct.

Authors

I S Savitskaya¹, D H Shokatayeva¹, A S Kistaubayeva¹, L V Ignatova¹, I E Digel²

Affiliations

¹ Al-Farabi Kazakh National University, Al-Farabi av. 71, Almaty, 050038, Kazakhstan.
² Aachen University of Applied Sciences, Heinrich-Mußmann-Street 1, Jülich, 52428, Germany.

Abstract

A biocomposite composed of bacterial cellulose (BC) gel-film and Bacillus subtilis (BS) cells was obtained and characterized with a view to future biomedical applications. The inclusion of functional ingredient (10¹⁰/g viable BS cells) in the composite was carried out by their joint aggregation with the BC gel-film. Immobilized BS cells displayed high antagonistic activity towards causative agents of wound infections such as Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, Pseudomonas aeruginosa. Application of the BC/BS-biocomposite for the treatment of excision wounds, performed on laboratory animals, stimulated reparative processes and shortened the healing time. Possible mechanisms of the wound-healing effect of BC/BS gel films are discussed. In this work we claim that the developed BC/BS-material can be positioned as a universal wound coating and sanitary-hygienic product.

Keywords: Bacillus subtilis; Bacterial cellulose; Biocomposite; Biotechnology; Microbial biotechnology; Microbiology; Wound healing.