Datasets: Sensitivity and protein digestion course of proteomic Filter Aided Sample Preparation

Jacek R Wiśniewski; Katharina Zettl

doi:10.1016/j.dib.2019.104530

Datasets: Sensitivity and protein digestion course of proteomic Filter Aided Sample Preparation

Data Brief. 2019 Sep 16:26:104530. doi: 10.1016/j.dib.2019.104530. eCollection 2019 Oct.

Authors

Jacek R Wiśniewski¹, Katharina Zettl¹

Affiliation

¹ Max Planck Institute of Biochemistry, 82152, Martinsried, Germany.

Abstract

Sensitivity of FASP was tested using SDS lysates from HeLa cells and mouse brain. Peptides were analyzed using a QExactive HF-X instrument. Whole cell lysates of Hela cells were processed with FASP using single or double, consecutive or successive, digestion with LysC or trypsin. The generated peptides were analyzed using a LTQ-Orbitrap mass spectrometer. These datasets accompany "Filter Aided Sample Preparation - A Tutorial" (Wiśniewski, 2019).

Keywords: Filter Aided Sample Preparation; Protein cleavage; Proteomic sample preparation; Shotgun proteomics.