Characterisation of 2-HP-β-cyclodextrin-PLGA nanoparticle complexes for potential use as ocular drug delivery vehicles

Fan Li; Yuqin Wen; Yan Zhang; Kangyu Zheng; Junfeng Ban; Qingchun Xie; Yifeng Wen; Qing Liu; Fohua Chen; Zhenjie Mo; Lizhong Liu; Yanzhong Chen; Zhufen Lu

doi:10.1080/21691401.2019.1683567

Characterisation of 2-HP-β-cyclodextrin-PLGA nanoparticle complexes for potential use as ocular drug delivery vehicles

Artif Cells Nanomed Biotechnol. 2019 Dec;47(1):4097-4108. doi: 10.1080/21691401.2019.1683567.

Authors

Fan Li^{1

2

3}, Yuqin Wen^{1

2

3}, Yan Zhang^{1

2

3}, Kangyu Zheng^{1

2

3}, Junfeng Ban^{1

2

3}, Qingchun Xie^{1

2

3}, Yifeng Wen^{1

2

3}, Qing Liu^{1

2

3}, Fohua Chen^{1

2

3}, Zhenjie Mo^{1

2

3}, Lizhong Liu⁴, Yanzhong Chen^{1

2

3}, Zhufen Lu^{1

2

3}

Affiliations

¹ Guangdong Provincial Key Laboratory of Advanced Drug Delivery Systems, Guangdong Pharmaceutical University, Guangzhou, People's Republic of China.
² Guangdong Provincial Engineering Center of Topical Precise Drug Delivery Systems, Guangdong Pharmaceutical University, Guangzhou, People's Republic of China.
³ R&D Team for Formulation Innovation, Guangdong Pharmaceutical University, Guangzhou, People's Republic of China.
⁴ Department of Hospital Pharmacy, Ningbo 7 Hospital, Ningbo, People's Republic of China.

PMID: 31663388
DOI: 10.1080/21691401.2019.1683567

Abstract

Aim: 2-HP-β-cyclodextrin-PLGA nanoparticle complexes were prepared to enhance the aqueous humour delivery of Triamcinolone acetonide.Materials & methods: Drug-loaded 2-HP-β-CD/PLGA nanoparticle complexes prepared by adapting a quasi-emulsion solvent evaporation technique. In vitro drug release, in vitro transcorneal permeation study, histopathological study and in vivo transcorneal penetration of PLGA nanoparticles and 2-HP-β-CD/PLGA nanoparticle complexes were evaluated. Results: Particle size distributions of 2-HP-β-CD/PLGA nanoparticle complexes were 149.4 ± 3.7 nm and presented stable system. Corneal penetration studies revealed steady sustained drug release (First-order); 2-HP-β-CD/PLGA nanoparticle complexes increased ocular bioavailability by increasing dispersion in the tear film and improving drug release. Conclusion: 2-HP-β-CD/PLGA nanoparticle complex formulation is a promising alternative to conventional eye drops.

Keywords: 2-hydroxypropyl-beta-cyclodextrin; Hydrophilicity; PLGA nanoparticles; ocular drug delivery; triamcinolone acetonide.

MeSH terms

2-Hydroxypropyl-beta-cyclodextrin / chemistry*
Animals
Cornea / metabolism
Drug Carriers / chemistry*
Drug Carriers / metabolism
Drug Liberation
Eye / metabolism*
Nanoparticles / chemistry*
Polylactic Acid-Polyglycolic Acid Copolymer / chemistry*
Rabbits

Substances

Drug Carriers
2-Hydroxypropyl-beta-cyclodextrin
Polylactic Acid-Polyglycolic Acid Copolymer