PDE4B gene polymorphism in Russian patients with panic disorder

Alena V Malakhova; Olga I Rudko; Vladimir V Sobolev; Artemii V Tretiakov; Elena A Naumova; Zarema G Kokaeva; Julia E Azimova; Eugene A Klimov

doi:10.3934/genet.2019.3.55

PDE4B gene polymorphism in Russian patients with panic disorder

AIMS Genet. 2019 Aug 20;6(3):55-63. doi: 10.3934/genet.2019.3.55. eCollection 2019.

Authors

Alena V Malakhova¹, Olga I Rudko¹, Vladimir V Sobolev^{2

3

4}, Artemii V Tretiakov¹, Elena A Naumova¹, Zarema G Kokaeva¹, Julia E Azimova^{5

6}, Eugene A Klimov^{1

3

7}

Affiliations

¹ Lomonosov Moscow State University, Faculty of Biology, Moscow, Russia.
² I.I. Mechnikov Research Institute for Vaccines and Sera, Laboratory of molecular immunology, Moscow, Russia.
³ University Diagnostic Laboratory LLC, Moscow, Russia.
⁴ Centre of Theoretical Problems of Physico-Chemical Pharmacology, Laboratory of Physicochemical and Genetic Problems of Dermatology, Russian Academy of Sciences, Moscow, Russia.
⁵ University headache clinic LLC, Moscow, Russia.
⁶ The Institute of General Pathology and Pathophysiology, Laboratory of Fundamental and Applied Pain Problems, Moscow, Russia.
⁷ Center of Experimental Embryology and Reproductive Biotechnologies, Russian Academy of Sciences, Moscow, Russia.

Abstract

Background: Panic disorder is a complex disease of unclear etiology but with an apparent genetic component. PDE4B gene product is involved in many cell processes owing to its function-regulation of the level of a second messenger cAMP. PDE4B gene polymorphism has been shown to be associated with some mental disorders including panic disorder.

Aims: The goal of our study was to evaluate the role of 3 SNPs in the PDE4B gene in the development of panic disorder.

Methods: 94 patients diagnosed with panic disorder according to the DSM-IV criteria were enrolled in the study. The population control group included 192 subjects. Genotyping was carried out by real-time PCR with TaqMan probes.

Results: The investigated substitutions are not associated with panic disorder in general and in female/male cohorts (p > 0.05). The analysis of complex genotypes demonstrated two protective complex genotypes (rs1040716:A, T + rs10454453:A + rs502958:A and rs1040716:A, T + rs502958:A) associated with panic disorder in general regardless of the patient's gender (p < 0.05). These genotypes did not correlate with the patient's sex.

Conclusions: We found two complex protective genotypes associated with panic disorder. This can be due to the fact that predisposition to the disease are associated with other genes, while PDE4B gene polymorphism reduces their effect.

Keywords: PDE4B gene; genetic association; panic disorder; rs1040716; rs10454453; rs502958.