ATP Measurement in Cerebrospinal Fluid Using a Microplate Reader

Methods Mol Biol. 2020:2041:233-241. doi: 10.1007/978-1-4939-9717-6_17.

Abstract

Imbalance in extracellular ATP levels in brain tissue has been suggested as a triggering factor for several neurological disorders. Here, we describe the most sensitive and reliable technique for monitoring the ATP levels in mice cerebrospinal samples collected by cisterna magna puncture technique and quantified using a microplate reader.

Keywords: ATP; Cerebrospinal fluid; In vivo; Luciferase; Luciferin; Microplate reader; Neurological disorders.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine Triphosphate / cerebrospinal fluid*
  • Animals
  • Brain / metabolism*
  • Cisterna Magna / metabolism*
  • Cisterna Magna / surgery
  • Luciferases / metabolism*
  • Mice
  • Microtechnology / methods*
  • Photometry / methods*

Substances

  • Adenosine Triphosphate
  • Luciferases