LncRNA H19 over-expression inhibited Th17 cell differentiation to relieve endometriosis through miR-342-3p/IER3 pathway

Zheying Liu; Liya Liu; Yun Zhong; Mingbo Cai; Junbi Gao; Chaoyue Tan; Xiaoxiao Han; Ruixia Guo; Liping Han

doi:10.1186/s13578-019-0346-3

LncRNA H19 over-expression inhibited Th17 cell differentiation to relieve endometriosis through miR-342-3p/IER3 pathway

Cell Biosci. 2019 Oct 15:9:84. doi: 10.1186/s13578-019-0346-3. eCollection 2019.

Authors

Zheying Liu^#¹, Liya Liu^#¹, Yun Zhong¹, Mingbo Cai¹, Junbi Gao¹, Chaoyue Tan¹, Xiaoxiao Han¹, Ruixia Guo¹, Liping Han¹

Affiliation

¹ Department of Gynecology, The First Affiliated Hospital of Zhengzhou University, No. 1 Jianshe Dong Road, Zhengzhou, 450052 People's Republic of China.

^# Contributed equally.

Abstract

Objective: To investigate the mechanism of LncRNA H19 in Th17 cell differentiation and endometrial stromal cells (ESCs) proliferation in endometriosis (EMS).

Methods: LncRNA H19, miR-342-3p and IER3 expressions were detected by qRT-PCR and western blot. The percentage of Th17 cells/CD4+ T cells was detected by flow cytometry. IL-17 level was measured by ELISA. The interaction of miR-342-3p and IER3 was confirmed by Luciferase reporter assay.

Results: LncRNA H19 and IER3 expressions were down-regulated in mononuclear cells from peritoneal fluid (PFMCs) of patients with EMS or under Th17 differentiation conditions, whereas miR-342-3p expression was up-regulated and the percentage of Th17 cells was increased in PFMCs of patients with EMS or under Th17 differentiation conditions. Over-expression of LncRNA H19 decreased IL-17 level and the percentage of Th17 cells/CD4+ T cells. Besides, we confirmed that miR-342-3p could target to IER3 and negatively regulate IER3 expression. LncRNA H19 over-expression suppressed Th17 differentiation and ESC proliferation through regulating miR-342-3p/IER3. In vivo experiments showed LncRNA H19 over-expression suppressed the growth of Th17 cell differentiation-induced endometriosis-like lesions.

Conclusion: LncRNA H19 was down-regulated in PFMC of patients with EMS or under Th17 polarizing conditions, and LncRNA H19 over-expression suppressed Th17 cell differentiation and ESCs proliferation through miR-342-3p/IER3 pathway.

Keywords: Endometriosis; IER3; LncRNA H19; Th17 cell differentiation; miR-342-3p.