Structural analysis of the lipoteichoic acid anchor glycolipid: Comparison of methods for degradation of the glycerophosphate backbone polymer

Tsukasa Shiraishi; Soh Yamamoto; Shin-Ichi Yokota

doi:10.1016/j.mimet.2019.105726

Structural analysis of the lipoteichoic acid anchor glycolipid: Comparison of methods for degradation of the glycerophosphate backbone polymer

J Microbiol Methods. 2019 Nov:166:105726. doi: 10.1016/j.mimet.2019.105726. Epub 2019 Oct 17.

Authors

Tsukasa Shiraishi¹, Soh Yamamoto¹, Shin-Ichi Yokota²

Affiliations

¹ Department of Microbiology, Sapporo Medical University School of Medicine, Minami-1 Nishi-17, Chuo-ku, Sapporo, Hokkaido 060-8556, Japan.
² Department of Microbiology, Sapporo Medical University School of Medicine, Minami-1 Nishi-17, Chuo-ku, Sapporo, Hokkaido 060-8556, Japan. Electronic address: syokota@sapmed.ac.jp.

PMID: 31629911
DOI: 10.1016/j.mimet.2019.105726

Abstract

Acetic acid treatment [98% (v/v), 100 °C, 3 h] was proposed as a new method for degrading the glycerophosphate polymer moiety of Gram-positive bacterial lipoteichoic acid. We demonstrated that this method resulted in partial O-acetylation on the carbohydrate residues of the anchor glycolipid. Hence, the acetic acid treatment is not suitable for the chemical structural analysis of lipoteichoic acid.

Keywords: Acetic acid; Anchor glycolipid; Hydrofluoric acid; Lipoteichoic acid; MALDI-TOF MS; O-acetylation.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Acetic Acid / chemistry*
Glycerophosphates / chemistry*
Glycolipids / chemistry*
Lactobacillus gasseri / metabolism
Lipopolysaccharides / chemistry*
Teichoic Acids / chemistry*

Substances

Glycerophosphates
Glycolipids
Lipopolysaccharides
Teichoic Acids
lipoteichoic acid
Acetic Acid