Continuous production of algicidal compounds against Akashiwo sanguinea via a Vibrio sp. co-culture

Yue Wang; Shuangfei Li; Guozhu Liu; Xiaoqiang Li; Qixia Yang; Ying Xu; Zhangli Hu; Chun-Yen Chen; Jo-Shu Chang

doi:10.1016/j.biortech.2019.122246

Continuous production of algicidal compounds against Akashiwo sanguinea via a Vibrio sp. co-culture

Bioresour Technol. 2020 Jan:295:122246. doi: 10.1016/j.biortech.2019.122246. Epub 2019 Oct 11.

Authors

Yue Wang¹, Shuangfei Li², Guozhu Liu², Xiaoqiang Li¹, Qixia Yang¹, Ying Xu², Zhangli Hu², Chun-Yen Chen³, Jo-Shu Chang⁴

Affiliations

¹ School of Materials and Environmental Engineering, Yantai University, Yantai, China.
² College of Life Sciences and Oceanography, Shenzhen University, Shenzhen, China.
³ University Center for Bioscience and Biotechnology, National Cheng Kung University, Tainan 701, Taiwan.
⁴ Department of Chemical and Materials Engineering, College of Engineering, Tunghai University, Taichung 407, Taiwan; Department of Chemical Engineering, National Cheng Kung University, Tainan 701, Taiwan; Center for Nanotechnology, Tunghai University, Taichung 407, Taiwan. Electronic address: changjs@mail.ncku.edu.tw.

PMID: 31629286
DOI: 10.1016/j.biortech.2019.122246

Abstract

Using biological treatment to deal with harmful algal blooms is highly potential over the physical and chemical methods due to its species specificity and eco-friendly characteristics. In this study, algicidal broth were produced from a Vibrio sp. co-culture composed mainly of V. brasilliensis and V. tubiashii. The productivity of the algicidal compounds was optimized under a dilution rate of 0.1 h^-1 with a minimum algicidal broth dosage of 0.3% for 100% algal lysis. The algicidal threshold and EC₅₀ of the spray-dried algicidal broth were 0.17 and 0.68 g/L, respectively. Treatment with the algicidal agents led to an increase in cellular reactive oxygen species (ROS) level that causes membrane damage as supported by the increase in Malondialdehyde (MDA) levels. and a further inhibition to the antioxidant system as indicated by a sharp decrease in the catalase (CAT) activity. The algicidal compound was identified as hexahydro pyrrolo[1,2-a] pyr azine-1,4-dione.

Keywords: Akashiwo sanguinea; Algicidal activity; Continuous culture; Oxidative stress; Vibrio.

MeSH terms

Coculture Techniques
Dinoflagellida*
Harmful Algal Bloom
Malondialdehyde
Vibrio*

Substances

Malondialdehyde