Tributyltin reduces bone mineral density by reprograming bone marrow mesenchymal stem cells in rat

Wenhuan Yao; Xinglong Wei; Hao Guo; Dong Cheng; Hui Li; Limin Sun; Shu'e Wang; Dongmei Guo; Yanli Yang; Jiliang Si

doi:10.1016/j.etap.2019.103271

Tributyltin reduces bone mineral density by reprograming bone marrow mesenchymal stem cells in rat

Environ Toxicol Pharmacol. 2020 Jan:73:103271. doi: 10.1016/j.etap.2019.103271. Epub 2019 Oct 7.

Authors

Wenhuan Yao¹, Xinglong Wei², Hao Guo², Dong Cheng¹, Hui Li¹, Limin Sun³, Shu'e Wang², Dongmei Guo², Yanli Yang⁴, Jiliang Si⁵

Affiliations

¹ Institute of Preventive Medicine, Shandong University, Jinan, China; Department of Toxicology, Shandong Center for Disease Control and Prevention, Jinan, China.
² Institute of Preventive Medicine, Shandong University, Jinan, China; Department of Environmental Health, School of Public Health, Shandong University, Jinan, China.
³ Orthopedics Department, Shandong Provincial Third Hospital, Jinan, China.
⁴ Department of Environmental Health, School of Public Health, Shandong University, Jinan, China.
⁵ Institute of Preventive Medicine, Shandong University, Jinan, China; Department of Environmental Health, School of Public Health, Shandong University, Jinan, China. Electronic address: sjlsdu@sdu.edu.cn.

PMID: 31627035
DOI: 10.1016/j.etap.2019.103271

Abstract

Tributyltin (TBT), a proven endocrine disrupter, was widely used in industry and agriculture. Previous research showed that TBT could alter the balance between osteogenesis and adipogenesis, which may have significant consequences for bone health. Herein, we exposed male rats to TBT chloride (TBTCl) to evaluate the deleterious effects of TBT on bone. Exposure to 50 μg kg^-1 TBT resulted in a significant decrease in bone mineral density (BMD) at the femur diaphysis region in the rat. A dose-dependent increase in lipid accumulation and adipocyte number was observed in the bone marrow (BM) of the femur. Meanwhile, TBTCl treatment significantly enhanced the expression of PPARγ and attenuated the expression of Runx2 and β-catenin in BM. In addition, serum ALP activity of TBT-exposed rats also showed a dose-dependent decrease. These results suggest that TBT could reduce BMD via inhibition of the Wnt/β-catenin pathway and skew the adipo-osteogenic balance in the BM of rats.

Keywords: Adipogenesis; Bone mineral density; Osteogenesis; Tributyltin; Wnt signal pathway.

MeSH terms

Animals
Bone Density / drug effects*
Environmental Pollutants / toxicity*
Male
Mesenchymal Stem Cells*
Rats
Trialkyltin Compounds / toxicity*

Substances

Environmental Pollutants
Trialkyltin Compounds
tributyltin