Complete Genome Sequence of a Virulent African Swine Fever Virus from a Domestic Pig in Ukraine

Ganna Kovalenko; Anne-Lise Ducluzeau; Liudmyla Ishchenko; Mykola Sushko; Maryna Sapachova; Nataliia Rudova; Oleksii Solodiankin; Anton Gerilovych; Ralf Dagdag; Matthew Redlinger; Maksym Bezymennyi; Maciej Frant; Christian E Lange; Inna Dubchak; Andrii A Mezhenskyi; Serhiy Nychyk; Eric Bortz; Devin M Drown

doi:10.1128/MRA.00883-19

Complete Genome Sequence of a Virulent African Swine Fever Virus from a Domestic Pig in Ukraine

Microbiol Resour Announc. 2019 Oct 17;8(42):e00883-19. doi: 10.1128/MRA.00883-19.

Authors

Ganna Kovalenko¹, Anne-Lise Ducluzeau², Liudmyla Ishchenko¹, Mykola Sushko³, Maryna Sapachova³, Nataliia Rudova⁴, Oleksii Solodiankin⁴, Anton Gerilovych⁴, Ralf Dagdag⁵, Matthew Redlinger⁵, Maksym Bezymennyi¹, Maciej Frant⁶, Christian E Lange⁷, Inna Dubchak⁸, Andrii A Mezhenskyi³, Serhiy Nychyk¹, Eric Bortz^{1

5}, Devin M Drown^{9

10}

Affiliations

¹ Institute of Veterinary Medicine (IVM), National Academy of Agrarian Sciences of Ukraine, Kiev, Ukraine.
² Institute of Arctic Biology, University of Alaska Fairbanks, Fairbanks, Alaska, USA.
³ State Scientific Research Institute of Laboratory Diagnostics and Veterinary and Sanitary Expertise (SSRILDVSE), Kiev, Ukraine.
⁴ National Scientific Center Institute of Experimental and Clinical Veterinary Medicine (NSC IECVM), Kharkiv, Ukraine.
⁵ Department of Biological Sciences, University of Alaska Anchorage, Anchorage, Alaska, USA.
⁶ National Veterinary Research Institute (NVRI), Pulawy, Poland.
⁷ Metabiota, San Francisco, California, USA.
⁸ Joint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, California, USA.
⁹ Institute of Arctic Biology, University of Alaska Fairbanks, Fairbanks, Alaska, USA dmdrown@alaska.edu.
¹⁰ Department of Biology and Wildlife, University of Alaska Fairbanks, Fairbanks, Alaska, USA.

Abstract

Here, we report the complete genome sequence of an African swine fever (ASF) virus (ASFV/Kyiv/2016/131) isolated from the spleen of a domestic pig in Ukraine with a lethal case of African swine fever. Using only long-read Nanopore sequences, we assembled a full-length genome of 191,911 base pairs in a single contig.

Grants and funding

P20 GM103395/GM/NIGMS NIH HHS/United States