We report on the new technical realization of single-shot color digital holographic microscopy. For this application, we propose to use an original three-wavelength red, green, and blue laser based on the Nd:YAG active element with sequential parametric downconversion and upconversion of optical frequencies into red (634 nm), green (532 nm), and blue (451 nm) spectral intervals. This light source provides high-power short (∼10 ns) pulses and enables simultaneous formation of three color interference patterns in a two-path interferometer. Their registration by a color image sensor provides fast acquisition of phase delay distribution induced by the inspected object at three wavelengths without spectral tuning.