More precise, more universal and more specific - the next generation of RNA-guided endonucleases for genome editing

FEBS J. 2019 Dec;286(23):4657-4660. doi: 10.1111/febs.15079. Epub 2019 Oct 14.

Abstract

CRISPR is a prokaryotic defence system that was adapted as a tool for genome editing and has become one of the most important discoveries of this century. CRISPR-associated endonucleases cleave DNA at precise sites, which are marked by complementary short-guided RNA. The recently developed versions of endonucleases are compatible with a broad range of PAM motifs, have a higher specificity and enable a specific nucleotide to be replaced.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Clustered Regularly Interspaced Short Palindromic Repeats / genetics*
  • DNA / genetics
  • Endonucleases / genetics
  • Endonucleases / metabolism
  • Gene Editing*
  • Humans
  • RNA, Guide, CRISPR-Cas Systems / genetics*

Substances

  • RNA, Guide, CRISPR-Cas Systems
  • DNA
  • Endonucleases