tRNA-derived fragments from the Sus scrofa tissues provide evidence of their conserved role in mammalian development

Marek Kazimierczyk; Agata Jędroszkowiak; Dorota Kowalczykiewicz; Maciej Szymański; Barbara Imiołczyk; Jerzy Ciesiołka; Jan Wrzesiński

doi:10.1016/j.bbrc.2019.10.062

tRNA-derived fragments from the Sus scrofa tissues provide evidence of their conserved role in mammalian development

Biochem Biophys Res Commun. 2019 Dec 10;520(3):514-519. doi: 10.1016/j.bbrc.2019.10.062. Epub 2019 Oct 11.

Authors

Marek Kazimierczyk¹, Agata Jędroszkowiak², Dorota Kowalczykiewicz¹, Maciej Szymański³, Barbara Imiołczyk¹, Jerzy Ciesiołka¹, Jan Wrzesiński⁴

Affiliations

¹ Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznań, Poland.
² Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznań, Poland; Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland.
³ Department of Computational Biology, Institute of Molecular Biology and Biotechnology, Adam Mickiewicz University, Poznań, Poland.
⁴ Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznań, Poland. Electronic address: wrzesinj@ibch.poznan.pl.

PMID: 31610915
DOI: 10.1016/j.bbrc.2019.10.062

Abstract

The recently discovered group of noncoding RNAs, which are fragments of tRNA molecules (tRFs), has not been fully characterized and its potential functions still require investigation. Porcine tRFs were characterized and compared to mouse and human tRFs. Two tRFs, 5' 32-33 nt and 3' 41-42 nt that are derived from the mature tRNAVal(CAC) and tRNAGly(GCC) were detected with the use of bioinformatics and the Northern blot method. The abundance of these tRFs in the case of Sus scrofa is restricted to the ovary and the kidney. The same tRFs were found in human cancer cells and in mouse sperm, circulating blood and its serum. The binding of selected sncRNAs (piRNA, 5'tRFVal(CAC) and miRNA) to the overexpressed PAZ domain of the PIWIL4 protein was also studied. It is noteworthy that porcine 5'tRFVal(CAC) and human 5'tRFVal(CAC)as well as 5'tRFGly(GCC) are bound to the PIWIL4 protein. The potential role of the analyzed tRFs in the development of mammals is also discussed.

Keywords: Mammalian tRNA fragments; Northern blot; PIWIL4 protein; Small RNA binding; Tissue specificity.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Argonaute Proteins / chemistry
Argonaute Proteins / genetics
Argonaute Proteins / metabolism
Conserved Sequence
Evolution, Molecular
Female
Humans
Male
Mammals / genetics*
Mammals / growth & development*
Mammals / metabolism
Mice
MicroRNAs / genetics
Models, Molecular
Nucleic Acid Conformation
Protein Binding
Protein Domains
Protein Structure, Tertiary
RNA, Small Interfering / genetics
RNA, Small Untranslated / chemistry
RNA, Small Untranslated / genetics
RNA, Small Untranslated / metabolism
RNA, Transfer / chemistry
RNA, Transfer / genetics*
RNA, Transfer / metabolism
RNA-Binding Proteins
Species Specificity
Sus scrofa / genetics*
Sus scrofa / growth & development*
Sus scrofa / metabolism

Substances

Argonaute Proteins
MicroRNAs
PIWIL4 protein, human
PIWIL4 protein, mouse
RNA, Small Interfering
RNA, Small Untranslated
RNA-Binding Proteins
RNA, Transfer