Core/shell Printing Scaffolds For Tissue Engineering Of Tubular Structures

J Vis Exp. 2019 Sep 27:(151). doi: 10.3791/59951.

Abstract

Three-dimensional (3D) printing of core/shell filaments allows direct fabrication of channel structures with a stable shell that is cross-linked at the interface with a liquid core. The latter is removed post-printing, leaving behind a hollow tube. Integrating an additive manufacturing technique (like the one described here with tailor-made [bio]inks, which structurally and biochemically mimic the native extracellular matrix [ECM]) is an important step towards advanced tissue engineering. However, precise fabrication of well-defined structures requires tailored fabrication strategies optimized for the material in use. Therefore, it is sensible to begin with a set-up that is customizable, simple-to-use, and compatible with a broad spectrum of materials and applications. This work presents an easy-to-manufacture core/shell nozzle with luer-compatibility to explore core/shell printing of woodpile structures, tested with a well-defined, alginate-based scaffold material formulation.

Publication types

  • Research Support, Non-U.S. Gov't
  • Video-Audio Media

MeSH terms

  • Alginates / chemistry*
  • Extracellular Matrix / chemistry*
  • Human Umbilical Vein Endothelial Cells / cytology*
  • Humans
  • Printing, Three-Dimensional / instrumentation*
  • Tissue Engineering / methods*
  • Tissue Scaffolds / chemistry*

Substances

  • Alginates