Characterization of Chromosome-Mediated BlaOXA-894 in Shewanella xiamenensis Isolated from Pig Wastewater

Huiyun Zou; Ziyu Zhou; Huiyu Xia; Qian Zhao; Xuewen Li

doi:10.3390/ijerph16193768

Characterization of Chromosome-Mediated Bla_OXA-894 in Shewanella xiamenensis Isolated from Pig Wastewater

Int J Environ Res Public Health. 2019 Oct 8;16(19):3768. doi: 10.3390/ijerph16193768.

Authors

Huiyun Zou¹, Ziyu Zhou², Huiyu Xia³, Qian Zhao⁴, Xuewen Li⁵

Affiliations

¹ Department of environment and health, School of Public Health, Shandong University, Jinan 250012, China. zouhuiyun@mail.sdu.edu.cn.
² Department of environment and health, School of Public Health, Shandong University, Jinan 250012, China. zhouziyu199622@mail.sdu.edu.cn.
³ Department of environment and health, School of Public Health, Shandong University, Jinan 250012, China. mosebyxhy@mail.sdu.edu.cn.
⁴ Department of environment and health, School of Public Health, Shandong University, Jinan 250012, China. 201835834@mail.sdu.edu.cn.
⁵ Department of environment and health, School of Public Health, Shandong University, Jinan 250012, China. lxw@sdu.edu.cn.

Abstract

A new variant of the bla_OXA-546 gene, namely bla_OXA-894, was identified on the chromosome of Shewanella xiamenensis isolated from pig wastewater in rural China. OXA-894 differs from OXA-546 (A46V, I219del) and OXA-48 (T167I, I219del) with two amino acid substitutions, respectively. The isolate was resistant to ampicillin, aztreonam, imipenem, meropenem and fosfomycin. Carba NP test confirmed S. xiamenensis strain sx20 as a carbapenemase-producer. The bla_OXA-894 gene was located between the gene encoding a LysR family transcriptional regulator and the C15 gene. Its gene environment was similar to other S. xiamenensis with chromosome-located bla_OXA-48-like genes. The T24H and T94V amino acid substitutions of LuxS protein were predicted to be deleterious, which may affect the virulence phenotype. The occurrence and potential health risk of carbapenem-resistant S. xiamenensis in a water environment is of concern.

Keywords: Shewanella xiamenensis; blaOXA-894; genetic environment; pig wastewater.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Bacterial Proteins / metabolism*
China
DNA, Bacterial / genetics*
Feces / microbiology*
Genetic Variation
Microbial Sensitivity Tests
Shewanella / genetics*
Swine / microbiology
Virulence / genetics*
Wastewater / microbiology*
beta-Lactamases / metabolism*

Substances

Bacterial Proteins
DNA, Bacterial
Waste Water
beta-Lactamases
carbapenemase