Wastewater streams are widely known to release silver-based nanoparticles (Ag-b-NPs) into the environment with a plethora of unknown consequences. Until recently, studies have commonly associated Ag-b-NP sources with products that contain these NPs for antimicrobial reasons, such as fabrics, cosmetics, and medical products. However, our study reveals that there is a thus far completely undocumented source of Ag-b-NPs: copper drinking water pipes. We applied cloud point extraction hyphenated to electrothermal atomic absorption spectrometry or single-particle inductively coupled plasma mass spectrometry to analyze the concentration and perform size-selective quantification of Ag-b-NPs in tap water passing through copper pipes. Up to 83 ng of total silver and 25 ng of Ag-b-NPs were present in tap water samples per liter, which resulted in an NP proportion of approximately 30% of total silver. In total, 96% of the measurable particle sizes ranged from 10 to 36 nm. Additionally, 53 μg of copper was released per liter tap water on average. The measurements included tap water from different sampling days and from four different buildings with varying ages, whereas Ag-b-NPs could be detected in the tap water of two buildings. Silver traces in the copper pipe material of 27.5 ± 4.4 μg g-1 were found to be responsible for the release of nanoparticulate silver into the tap water.