Anti-obesity effects of α-cyclodextrin-stabilized 4-methylthio-3-butenyl isothiocyanate from daikon (Raphanus sativus var. longipinnatus) in mice

Hinako Okamoto; Syoko Ino; Nanako Nihei; Naoko Ikuta; Chihiro Ueno; Aya Itoi; Yutaka Yoshikawa; Keiji Terao; Norihiro Sakamoto

doi:10.3164/jcbn.19-11

Anti-obesity effects of α-cyclodextrin-stabilized 4-methylthio-3-butenyl isothiocyanate from daikon (Raphanus sativus var. longipinnatus) in mice

J Clin Biochem Nutr. 2019 Sep;65(2):99-108. doi: 10.3164/jcbn.19-11. Epub 2019 Aug 9.

Authors

Hinako Okamoto^{1

2}, Syoko Ino³, Nanako Nihei², Naoko Ikuta¹, Chihiro Ueno², Aya Itoi^{3

4}, Yutaka Yoshikawa^{3

4}, Keiji Terao^{1

2}, Norihiro Sakamoto¹

Affiliations

¹ Department of Social/Community Medicine and Health Science, Food and Drug Evaluation Science, Graduate School of Medicine, Kobe University, 7-5-1 Kusunoki-cho, Chuo-ku, Kobe, Hyogo 650-0017, Japan.
² CycloChem Bio Co., Ltd., 7-4-5 Minatojima-minamimachi, Chuo-ku, Kobe, Hyogo 650-0047, Japan.
³ Department of Health, Sports, and Nutrition, Faculty of Health and Welfare, Kobe Woman's University, 4-7-2 Minatojima-nakamachi, Chuo-ku, Kobe, Hyogo 650-0046, Japan.
⁴ Graduate School of Science of Health and Nutrition, Kobe Woman's University, 4-7-2 Minatojima-nakamachi, Chuo-ku, Kobe, Hyogo 650-0046, Japan.

Abstract

4-Methylthio-3-butenyl isothiocyanate (MTBI) is a pungent bioactive constituent found in daikon. However, MTBI is immediately hydrolyzed to 3-hydroxy-methylene-2-thioxopyrrolidine in grated daikon. In this study, we evaluated whether MTBI in grated daikon complexed with α-cyclodextrin (αCD) has anti-obesity effects in mice. C57BL/6J mice were fed a normal diet (normal group), high-fat diet (HFD, control group), HFD with αCD (αCD group), or HFD with MTBI-αCD (MTBI-αCD group) for 16 weeks. The results showed that the final body weight, epididymal white adipose tissue weight, and plasma triglyceride and total cholesterol levels were significantly lower in the MTBI-αCD group than in the control group. The cell size in epididymal adipose tissue was significantly smaller and the accumulation of lipids in the liver was significantly lower in the MTBI-αCD group than in the control group. Furthermore, real-time polymerase chain reaction showed that the mRNA expression level of tumor necrosis factor-alpha was suppressed in the MTBI-αCD group. We also observed low superoxide dismutase activity in the MTBI-αCD group, possibly because MTBI-αCD has the potential to resist HFD-induced oxidative injury. In conclusion, MTBI-αCD exerted anti-inflammation and antioxidant effects to suppress lipid accumulation in epididymal adipose tissue and the liver. These effects then prevented HFD-induced obesity in mice.

Keywords: 4-methylthio-3-butenyl isothiocyanate; anti-obesity effect; antioxidant effect; high-fat diet; αCD.