Levan production from sucrose using chicken feather peptone as a low cost supplemental nutrient source

Bhuvaneshwari Veerapandian; Saravanan Ramiah Shanmugam; Srivathsan Varadhan; Kartik Kumar Sarwareddy; Krishna Priya Mani; V Ponnusami

doi:10.1016/j.carbpol.2019.115361

Levan production from sucrose using chicken feather peptone as a low cost supplemental nutrient source

Carbohydr Polym. 2020 Jan 1:227:115361. doi: 10.1016/j.carbpol.2019.115361. Epub 2019 Sep 23.

Authors

Bhuvaneshwari Veerapandian¹, Saravanan Ramiah Shanmugam¹, Srivathsan Varadhan¹, Kartik Kumar Sarwareddy², Krishna Priya Mani², V Ponnusami³

Affiliations

¹ Biomass conversion and Bioproducts Laboratory, Center for Bioenergy, School of Chemical & Biotechnology, SASTRA Deemed University, Thirumalaisamudram, Tamil Nadu, India.
² Cardiomyocyte Toxicity and Oncology Research Laboratory, School of Chemical & Biotechnology, SASTRA Deemed University, Thirumalaisamudram, Tamil Nadu, India.
³ Biomass conversion and Bioproducts Laboratory, Center for Bioenergy, School of Chemical & Biotechnology, SASTRA Deemed University, Thirumalaisamudram, Tamil Nadu, India. Electronic address: vponnu@chem.sastra.edu.

PMID: 31590858
DOI: 10.1016/j.carbpol.2019.115361

Abstract

Chicken feather peptone (CFP) derived from poultry waste is a rich source of essential minerals and amino acids. This, along with suitable carbon source, can be used as a low cost complex supplemental nutrient source for microbial fermentation. In the present work, CFP blended with sucrose was evaluated for the production of levan using Bacillus subtilis MTCC 441. Amount of CFP added to the medium significantly influenced levan production and it was found that at a concentration 2 g/L, maximum levan yield of 0.26 ± 0.04 g/g sucrose was obtained. The levan yield obtained with CFP as a low cost supplemental nutrient source was comparable with that obtained from commercial medium (0.31 ± 0.02 g/g sucrose). Levan produced using CFP was tested on primary cell lines at various concentrations (100-1000 μM) and found to be non-toxic and bio-compatible in nature. This indicates that CFP could be used as low cost nutrient source for levan production.

Keywords: Acid hydrolysis; Chicken feather peptone; Chicken feathers; Levan production.

MeSH terms

Animals
Bacillus subtilis / metabolism*
Cell Survival
Chickens
Feathers / chemistry
Fermentation
Fructans / metabolism*
Fructans / pharmacology
Human Umbilical Vein Endothelial Cells / drug effects
Humans
Peptones / metabolism*
Sucrose / metabolism*

Substances

Fructans
Peptones
Sucrose
levan