Highly efficient and selective antimicrobial isonicotinylhydrazide-coated polyoxometalate-functionalized silver nanoparticles

Akhela Umapathi; Navya P Nagaraju; Harishkumar Madhyastha; Devendra Jain; Sangly P Srinivas; Vincent M Rotello; Hemant Kumar Daima

doi:10.1016/j.colsurfb.2019.110522

Highly efficient and selective antimicrobial isonicotinylhydrazide-coated polyoxometalate-functionalized silver nanoparticles

Colloids Surf B Biointerfaces. 2019 Dec 1:184:110522. doi: 10.1016/j.colsurfb.2019.110522. Epub 2019 Sep 25.

Authors

Akhela Umapathi¹, Navya P Nagaraju², Harishkumar Madhyastha³, Devendra Jain⁴, Sangly P Srinivas⁵, Vincent M Rotello⁶, Hemant Kumar Daima⁷

Affiliations

¹ Amity Center for Nanobiotechnology and Nanomedicine (ACNN), Amity Institute of Biotechnology, Amity University Rajasthan, Kant Kalwar, NH-11C, Jaipur-Delhi Highway, Jaipur, 303002, Rajasthan, India.
² Nano-Bio Interfacial Research Laboratory (NBIRL), Department of Biotechnology, Siddaganga Institute of Technology, Tumkur, 572103, Karnataka, India; Department of Biotechnology, Bannari Amman Institute of Technology, Sathyamangalam, Erode - 638401, Tamil Nadu, India.
³ Department of Applied Physiology, Faculty of Medicine, University of Miyazaki, Miyazaki, 8891692, Japan.
⁴ Department of Molecular Biology and Biotechnology, Rajasthan College of Agriculture, Maharana Pratap University of Agriculture and Technology, Udaipur, 313001, Rajasthan, India.
⁵ School of Optometry, Indiana University, Bloomington, 47405, IN, USA.
⁶ Department of Chemistry, University of Massachusetts (UMass) Amherst, 710 North Pleasant Street, Amherst, 01003 MA, USA.
⁷ Amity Center for Nanobiotechnology and Nanomedicine (ACNN), Amity Institute of Biotechnology, Amity University Rajasthan, Kant Kalwar, NH-11C, Jaipur-Delhi Highway, Jaipur, 303002, Rajasthan, India; Nano-Bio Interfacial Research Laboratory (NBIRL), Department of Biotechnology, Siddaganga Institute of Technology, Tumkur, 572103, Karnataka, India. Electronic address: hkdaima@jpr.amity.edu.

PMID: 31586898
DOI: 10.1016/j.colsurfb.2019.110522

Abstract

With the rapidly approaching post-antibiotic era, new and effective combinations of antibiotics are imperative to combat multiple drug resistance (MDR). We have synthesized multimodal antimicrobials that integrate the antibiotic isonicotinylhydrazide (INH), silver nanoparticles (AgNPs^INH), and two different polyoxometalates (POMs) namely, phosphotungstic acid (PTA) and phosphomolybdic acid (PMA) to prepare AgNPs^INH@PTA and AgNPs^INH@PMA, respectively. AgNPs^INH have peroxidase-like (nanozyme) activity and very high antibacterial potential toward S. aureus, which was further enhanced upon modification with POMs. The selectivity of these functional nanozymes was evaluated with m5S mouse fibroblasts using WST-8, LDH viability, in vitro reactive oxygen species (ROS) generation assays, and crystal violet morphological studies. These investigations showed very low cytotoxicity for the nanoparticles compared to free metal ions (Ag⁺), pristine POMs and INH, demonstrating the ability of multifunctional materials to provide efficient and selective antimicrobials.

Keywords: Antimicrobial; Cytotoxicity; Isonicotinylhydrazide; Nanozyme; Polyoxometalates; Silver nanoparticles.

MeSH terms

Animals
Anti-Bacterial Agents / chemical synthesis
Anti-Bacterial Agents / chemistry
Anti-Bacterial Agents / pharmacology*
Isoniazid / chemistry
Isoniazid / pharmacology*
Metal Nanoparticles / chemistry*
Mice
Microbial Sensitivity Tests
Molecular Structure
Particle Size
Silver / chemistry
Silver / pharmacology*
Staphylococcus aureus / drug effects*
Surface Properties
Tungsten Compounds / chemistry
Tungsten Compounds / pharmacology*

Substances

Anti-Bacterial Agents
Tungsten Compounds
polyoxometalate I
Silver
Isoniazid