ERp29 inhibition attenuates TCA toxicity via affecting p38/p53- dependent pathway in human trophoblast HTR-8/SVeno cells

Arch Biochem Biophys. 2019 Nov 15:676:108125. doi: 10.1016/j.abb.2019.108125. Epub 2019 Oct 3.

Abstract

Intrahepatic cholestasis of pregnancy (ICP) is a liver disorder occurred in pregnant women, and the mechanism for such disease is still unclear. The bioinformatics analysis of our previous study has revealed the abnormal expression of endoplasmic reticulum protein 29 (ERp29) in placental tissue of ICP patients. In this study, the function of ERp29 was further explored using in vitro model of ICP. The results showed that up-regulation of ERp29 occurred in TCA (taurocholic acid)-treated human trophoblast HTR-8/SVeno cells, and ERp29 inhibition reversed TCA toxicity via attenuating G2/M arrest and cell apoptosis. Mechanical study revealed ERp29 inhibition suppressed phosphorylation and kinase activity of p38, thus subsequently affecting expression and phosphorylation of p53 (ser18) as well as the transcriptional activity of p53. The conduction of this study might confirm the important role of ERp29 in ICP and which would be helpful for the development of target therapeutic method for ICP.

Keywords: Endoplasmic reticulum protein 29; Intrahepatic cholestasis of pregnancy; p38/p53-dependent pathway.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Apoptosis / drug effects
  • Apoptosis / genetics
  • Cell Line
  • Cholestasis, Intrahepatic / genetics
  • Cholestasis, Intrahepatic / metabolism
  • Cholestasis, Intrahepatic / pathology
  • Female
  • G2 Phase Cell Cycle Checkpoints / drug effects
  • G2 Phase Cell Cycle Checkpoints / genetics
  • Gene Knockdown Techniques*
  • Heat-Shock Proteins / deficiency
  • Heat-Shock Proteins / genetics*
  • Humans
  • M Phase Cell Cycle Checkpoints / drug effects
  • M Phase Cell Cycle Checkpoints / genetics
  • Phosphorylation / drug effects
  • Phosphorylation / genetics
  • Pregnancy
  • Pregnancy Complications / genetics
  • Pregnancy Complications / metabolism
  • Pregnancy Complications / pathology
  • Taurocholic Acid / toxicity*
  • Trophoblasts / cytology*
  • Trophoblasts / drug effects
  • Trophoblasts / metabolism*
  • Tumor Suppressor Protein p53 / metabolism*
  • p38 Mitogen-Activated Protein Kinases / metabolism*

Substances

  • ERP29 protein, human
  • Heat-Shock Proteins
  • Tumor Suppressor Protein p53
  • Taurocholic Acid
  • p38 Mitogen-Activated Protein Kinases

Supplementary concepts

  • Intrahepatic Cholestasis of Pregnancy