Nanoceria as a DNase I mimicking nanozyme

Fang Xu; Qinwei Lu; Po-Jung Jimmy Huang; Juewen Liu

doi:10.1039/c9cc06782e

Nanoceria as a DNase I mimicking nanozyme

Chem Commun (Camb). 2019 Oct 31;55(88):13215-13218. doi: 10.1039/c9cc06782e.

Authors

Fang Xu¹, Qinwei Lu², Po-Jung Jimmy Huang³, Juewen Liu³

Affiliations

¹ Department of Pharmaceutical Engineering, School of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, China and Department of Chemistry, Waterloo Institute for Nanotechnology, University of Waterloo, Waterloo, Ontario, N2L 3G1, Canada. liujw@uwaterloo.ca.
² Department of Pharmaceutical Engineering, School of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, China.
³ Department of Chemistry, Waterloo Institute for Nanotechnology, University of Waterloo, Waterloo, Ontario, N2L 3G1, Canada. liujw@uwaterloo.ca.

PMID: 31577297
DOI: 10.1039/c9cc06782e

Abstract

We herein communicate the DNase I like activity of nanoceria (CeO2 nanoparticles). Both CeO2 and DNase I cleave polyadenine (poly-A) DNA down to ∼5-mer fragments as the major products, although further cleavage to even shorter fragments was observed with CeO2. Mass spectrometry indicates a hydrolytic cleavage mechanism instead of oxidative cleavage.

MeSH terms

Cerium / chemistry
Cerium / metabolism*
DNA / chemistry
DNA / metabolism*
Deoxyribonuclease I / chemistry
Deoxyribonuclease I / metabolism*
Hydrolysis
Mass Spectrometry
Nanoparticles / chemistry
Nanoparticles / metabolism*
Oxidation-Reduction
Poly A / chemistry
Poly A / metabolism*

Substances

polyadenine
Poly A
Cerium
ceric oxide
DNA
Deoxyribonuclease I