Co-administration of aspirin and adipose-derived stem cell conditioned medium improves the functional recovery of the optic pathway in a lysolecithin-induced demyelination model

Adel Galeshi; Maryam Ghasemi-Kasman; Farideh Feizi; Nahid Davoodian; Leila Zare; Zeinab Abedian

doi:10.2147/NDT.S218594

Co-administration of aspirin and adipose-derived stem cell conditioned medium improves the functional recovery of the optic pathway in a lysolecithin-induced demyelination model

Neuropsychiatr Dis Treat. 2019 Sep 17:15:2681-2694. doi: 10.2147/NDT.S218594. eCollection 2019.

Authors

Adel Galeshi^{1

2}, Maryam Ghasemi-Kasman^{3

4}, Farideh Feizi^{2

3}, Nahid Davoodian^{5

6}, Leila Zare⁷, Zeinab Abedian³

Affiliations

¹ Babol University of Medical Sciences, Babol, Iran.
² Department of Anatomical Sciences, Faculty of Medicine, Babol University of Medical Sciences, Babol, Iran.
³ Cellular and Molecular Biology Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran.
⁴ Neuroscience Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran.
⁵ Molecular Medicine Research Center, Hormozgan Health Institute, Hormozgan University of Medical Sciences, Bandar Abbas, Iran.
⁶ Department of Clinical Biochemistry, Faculty of Medicine, Hormozgan University of Medical Sciences, Bandar Abbas, Iran.
⁷ Department of Physiology, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran.

Abstract

Introduction: Based on beneficial effects of aspirin and mesenchymal stem cells (MSCs) on myelin repair, in a preset study, effects of co-administration of aspirin and conditioned medium from adipose tissue-derived stem cells (ADSC-CM) on functional recovery of optic pathway, demyelination levels, and astrocytes' activation were evaluated in a lysolecithin (LPC)-induced demyelination model of optic chiasm.

Methods: LPC (1%, 2 µL) was injected into the rat optic chiasm and animals underwent daily intraperitoneal (i.p.) injections of ADSCs-CM and oral gavage of aspirin at a dose of 25 mg/kg for 14 days post LPC injection. The conductivity of visual signals was assessed using visual evoked potential recordings (VEPs) before LPC injection and on days 7 and 14 post lesion. Immunostaining against PDGFRα as oligodendrocyte precursor cells marker, MOG as mature myelin marker, and GFAP as astrocyte marker was performed on brain sections at day 14 post LPC injection. FluoroMyelin staining was also used to measure the extent of demyelination areas.

Results: Our results showed that administration of ADSCs-CM and aspirin significantly reduced the latency of VEP waves in LPC receiving animals. In addition, demyelination levels and GFAP expressing cells were attenuated while the number of oligodendrocyte precursor cells significantly increased in rats treated with ADSCs-CM and aspirin.

Conclusion: Overall, our results suggest that co-administration of ADSCs-CM and aspirin improves the functional recovery of optic pathway through amelioration of astrocyte activation and attenuation of demyelination level.

Keywords: aspirin; conditioned medium; demyelination; lysolecithin; mesenchymal stem cells; optic chiasm.