Testing the Clinical Applicability of Resin Infiltration of Developmental Enamel Hypomineralization Lesions Using an In Vitro Model

Dagmar Schnabl; Viktoria Dudasne-Orosz; Rudolf Glueckert; Stephan Handschuh; Ines Kapferer-Seebacher; Herbert Dumfahrt

doi:10.5005/jp-journals-10005-1609

Testing the Clinical Applicability of Resin Infiltration of Developmental Enamel Hypomineralization Lesions Using an In Vitro Model

Int J Clin Pediatr Dent. 2019 Mar-Apr;12(2):126-132. doi: 10.5005/jp-journals-10005-1609.

Authors

Dagmar Schnabl¹, Viktoria Dudasne-Orosz¹, Rudolf Glueckert², Stephan Handschuh³, Ines Kapferer-Seebacher¹, Herbert Dumfahrt¹

Affiliations

¹ University Hospital of Dental Prosthetics and Restorative Dentistry, Medical University of Innsbruck, Innsbruck, Austria.
² Department of Otorhinolaryngology, Medical University of Innsbruck, Innsbruck, Austria.
³ VetCore Imaging Unit, Veterinary University of Vienna, Vienna, Austria.

Abstract

Aim: The aim of this study is to investigate the penetration abilities of a commercially available low-viscosity resin infiltrant into developmentally hypomineralized teeth in vitro.

Materials and methods: Four extracted third molars of a 17-year-old patient with signs of developmental enamel hypomineralization (discoloration, increased opacity, and surface roughness) were infiltrated with a low-viscosity resin mixed with a fluorescent dye, according to the manufacturer's standard protocol. Four extracted molars with sound enamel or showing only initial fissure caries were used as a control group. Specimens were embedded in polymethylmethacrylate, and grindings were prepared. High-resolution projectional radiography of the grindings was performed, and, for one specimen, quantitative micro-computed tomography was used to measure hydroxyapatite density in enamel and dentin lesions. After decalcification, the grindings were examined by reflected bright-field microscopy, wide-field fluorescence microscopy, and confocal laser scanning microscopy. Fluorescence micrographs were superimposed on the radiographs and analyzed correlatively.

Results: The pattern of hypo-/demineralization in enamel and dentin in developmentally hypomineralized teeth showed a good congruence with the pattern of resin infiltration. Cavitations and dentin tubules up to a depth of 2 mm beyond cavitations were filled by the infiltrant. In control teeth, the penetration of the infiltrant was limited to decalcified enamel areas (initial fissure caries).

Conclusions: In vitro infiltration of developmentally hypomineralized enamel was successful.

Clinical significance: Resin infiltration might be considered as a routine procedure in the treatment of developmentally hypomineralized teeth. Further investigations with higher sample sizes, different degrees of severity, different stages of lesion extension, and modified treatment protocols are necessary.

How to cite this article: Schnabl D, Dudasne-Orosz V, et al. Testing the Clinical Applicability of Resin Infiltration of Developmental Enamel Hypomineralization Lesions Using an In Vitro Model. Int J Clin Pediatr Dent 2019;12(2):126-132.

Keywords: Cheese molars; Developmental enamel hypomineralization; Laboratory research; Low-viscosity resin; Penetration depth; Resin infiltration.