Demonstration of the broad spectrum in vitro activity of finafloxacin against pathogens of biodefence interest

Kay B Barnes; Steven D Zumbrun; Stephanie A Halasohoris; Purvi D Desai; Lynda L Miller; Mark I Richards; Paul Russell; Christine Bentley; Sarah V Harding

doi:10.1128/AAC.01470-19

Demonstration of the broad spectrum in vitro activity of finafloxacin against pathogens of biodefence interest

Antimicrob Agents Chemother. 2019 Sep 9;63(12):e01470-19. doi: 10.1128/AAC.01470-19. Epub 2019 Sep 30.

Authors

Kay B Barnes¹, Steven D Zumbrun², Stephanie A Halasohoris², Purvi D Desai², Lynda L Miller², Mark I Richards¹, Paul Russell¹, Christine Bentley³, Sarah V Harding⁴

Affiliations

¹ Defence Science and Technology Laboratory, Porton Down, Salisbury, UK.
² United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, Maryland, USA.
³ MerLion Pharmaceuticals, Berlin, Germany.
⁴ Defence Science and Technology Laboratory, Porton Down, Salisbury, UK svharding@dstl.gov.uk.

Abstract

This study investigated the in vitro activity of finafloxacin against panels of the biodefence pathogens. Broth microdilution assays were performed at neutral and acidic pH, to determine the effectiveness of the antibiotics in conditions typical of an intracellular environment. In all instances, finafloxacin demonstrated superior activity at low pH. These results highlight the importance of evaluating antimicrobial efficacy in conditions relevant to those encountered in vivo.