Acute phase proteins, proinflammatory cytokines and oxidative stress biomarkers in sheep, goats and she-camels with Coxiella burnetii infection-induced abortion

Wael El-Deeb; Ibrahim Ghoneim; Mahmoud Fayez; Ibrahim Elsohaby; Abdulrahman Alhaider; Magdy ElGioushy

doi:10.1016/j.cimid.2019.101352

Acute phase proteins, proinflammatory cytokines and oxidative stress biomarkers in sheep, goats and she-camels with Coxiella burnetii infection-induced abortion

Comp Immunol Microbiol Infect Dis. 2019 Dec:67:101352. doi: 10.1016/j.cimid.2019.101352. Epub 2019 Sep 24.

Authors

Wael El-Deeb¹, Ibrahim Ghoneim², Mahmoud Fayez³, Ibrahim Elsohaby⁴, Abdulrahman Alhaider⁵, Magdy ElGioushy⁶

Affiliations

¹ Department of Clinical Sciences, College of Veterinary Medicine, King Faisal University, Al-Ahsa 31982, Al-Hofuf P.O. 400, Saudi Arabia; Department of Veterinary Medicine, Infectious Diseases and Fish Diseases, Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt. Electronic address: weldeeb@kfu.edu.sa.
² Department of Clinical Sciences, College of Veterinary Medicine, King Faisal University, Al-Ahsa 31982, Al-Hofuf P.O. 400, Saudi Arabia; Department of Theriogenology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt.
³ Ministry of Agriculture, Al Ahsa Veterinary Diagnostic Laboratory, Saudi Arabia; Veterinary Serum and Vaccine Research Institute, Ministry of Agriculture, Cairo, Egypt.
⁴ Department of Animal Medicine, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt; Department of Health Management, Atlantic Veterinary College, University of Prince Edward Island, Charlottetown, PEI, Canada.
⁵ Department of Clinical Sciences, College of Veterinary Medicine, King Faisal University, Al-Ahsa 31982, Al-Hofuf P.O. 400, Saudi Arabia.
⁶ Department of Animal Medicine, Faculty of Veterinary Medicine, Aswan University, Aswan, Egypt.

PMID: 31568899
DOI: 10.1016/j.cimid.2019.101352

Abstract

Acute phase proteins (APPs) and oxidative stress are helpful markers in diagnosis of several infectious diseases. APPs, proinflammatory cytokines and oxidative stress markers were evaluated for their role in the diagnosis of naturally acquired Coxiella burnetii (Q fever) associated with abortion in sheep, goats and she-camels. Blood, aborted materials and vaginal swabs were collected from mixed herds in the Eastern Province of Saudi Arabia. Antioxidant biomarkers showed significant decline in cases of abortion compared to control animals at delivery time. The correlation between disease status and all parameters ranged from moderate to high. The APPs, cytokines and the oxidative stress marker malondialdehyde (MDA) displayed a high degree of distinction between aborted sheep and goat and normal delivered animals (AUC > 0.90). However, only MDA showed a high degree of differentiation (AUC > 0.90) between aborted she-camels and normal delivered controls. In conclusion, results from our study allow us to recommend using APPs, cytokines and oxidative stress markers as an additional tool for diagnosis of naturally occurring C. burnetii infection in sheep, goats and she-camels. However, it does not replace standard procedures for detection of C. burnetii.

Keywords: Abortion; Cytokines; Haptoglobin; Malondialdehyde; Q fever; Serum amyloid A.

MeSH terms

Abortion, Veterinary / microbiology*
Animals
Antibodies, Bacterial / blood
Camelus
Coxiella burnetii / genetics*
Coxiella burnetii / isolation & purification
Cytokines / blood*
DNA, Bacterial / genetics
Female
Goat Diseases / diagnosis
Goat Diseases / microbiology
Goat Diseases / pathology
Goats
Malondialdehyde / analysis
Oxidative Stress / physiology
Pregnancy
Q Fever / diagnosis
Q Fever / pathology
Q Fever / veterinary*
Saudi Arabia
Serum Amyloid A Protein / analysis*
Sheep
Sheep Diseases / diagnosis
Sheep Diseases / microbiology
Sheep Diseases / pathology

Substances

Antibodies, Bacterial
Cytokines
DNA, Bacterial
Serum Amyloid A Protein
Malondialdehyde