Lotus seed oligosaccharides at various dosages with prebiotic activity regulate gut microbiota and relieve constipation in mice

Han Su; Jinghao Chen; Song Miao; Kaibo Deng; Jiawen Liu; Shaoxiao Zeng; Baodong Zheng; Xu Lu

doi:10.1016/j.fct.2019.110838

Lotus seed oligosaccharides at various dosages with prebiotic activity regulate gut microbiota and relieve constipation in mice

Food Chem Toxicol. 2019 Dec:134:110838. doi: 10.1016/j.fct.2019.110838. Epub 2019 Sep 27.

Authors

Han Su¹, Jinghao Chen¹, Song Miao², Kaibo Deng³, Jiawen Liu⁴, Shaoxiao Zeng⁵, Baodong Zheng⁶, Xu Lu⁷

Affiliations

¹ College of Food Science, Fujian Agriculture and Forestry University, 15 Shangxiadian Road, 350002, Fuzhou, China; China-Ireland International Cooperation Center for Food Material Science and Structure Design, Fujian Agriculture and Forestry University, 350002, Fuzhou, China; Fujian Provincial Key Laboratory of Quality Science and Processing Technology in Special Starch, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
² Teagasc Food Research Centre, Food Chemistry and Technology Department, Moorepark, Fermoy, Co.Cork, Ireland; College of Food Science, Fujian Agriculture and Forestry University, 15 Shangxiadian Road, 350002, Fuzhou, China; China-Ireland International Cooperation Center for Food Material Science and Structure Design, Fujian Agriculture and Forestry University, 350002, Fuzhou, China.
³ College of Food Science, Fujian Agriculture and Forestry University, 15 Shangxiadian Road, 350002, Fuzhou, China; Teagasc Food Research Centre, Food Chemistry and Technology Department, Moorepark, Fermoy, Co.Cork, Ireland; Institute of Food Science and Technology, Fujian Agriculture and Forestry University, 18 Simon Pit Road, 350002, Fuzhou, China; China-Ireland International Cooperation Center for Food Material Science and Structure Design, Fujian Agriculture and Forestry University, 350002, Fuzhou, China; Fujian Provincial Key Laboratory of Quality Science and Processing Technology in Special Starch, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
⁴ College of Food Science, Fujian Agriculture and Forestry University, 15 Shangxiadian Road, 350002, Fuzhou, China.
⁵ College of Food Science, Fujian Agriculture and Forestry University, 15 Shangxiadian Road, 350002, Fuzhou, China; Institute of Food Science and Technology, Fujian Agriculture and Forestry University, 18 Simon Pit Road, 350002, Fuzhou, China; China-Ireland International Cooperation Center for Food Material Science and Structure Design, Fujian Agriculture and Forestry University, 350002, Fuzhou, China; Fujian Provincial Key Laboratory of Quality Science and Processing Technology in Special Starch, Fujian Agriculture and Forestry University, Fuzhou, 350002, China. Electronic address: zsxfst@163.com.
⁶ College of Food Science, Fujian Agriculture and Forestry University, 15 Shangxiadian Road, 350002, Fuzhou, China; Institute of Food Science and Technology, Fujian Agriculture and Forestry University, 18 Simon Pit Road, 350002, Fuzhou, China; China-Ireland International Cooperation Center for Food Material Science and Structure Design, Fujian Agriculture and Forestry University, 350002, Fuzhou, China; Fujian Provincial Key Laboratory of Quality Science and Processing Technology in Special Starch, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
⁷ College of Food Science, Fujian Agriculture and Forestry University, 15 Shangxiadian Road, 350002, Fuzhou, China; Teagasc Food Research Centre, Food Chemistry and Technology Department, Moorepark, Fermoy, Co.Cork, Ireland; Institute of Food Science and Technology, Fujian Agriculture and Forestry University, 18 Simon Pit Road, 350002, Fuzhou, China; China-Ireland International Cooperation Center for Food Material Science and Structure Design, Fujian Agriculture and Forestry University, 350002, Fuzhou, China; Fujian Provincial Key Laboratory of Quality Science and Processing Technology in Special Starch, Fujian Agriculture and Forestry University, Fuzhou, 350002, China. Electronic address: lxvfst@yeah.net.

PMID: 31568850
DOI: 10.1016/j.fct.2019.110838

Abstract

The aim of this study was to evaluate the effects of lotus seed oligosaccharides (formulation consisting of LSO2, LSO3-1, LSO3-2 and LSO4; relative ratios are 1.107:0.554:0.183:0.443, m/m/m/m) at dosages of 0.42, 0.83 g/d/kg bw and 2.49 g/d/kg bw on the microbiota composition and the propulsion of intestinal contents in the gut of mice. The results showed that fecal water content increased in treated mice; there was less gut microbiota diversity than in other groups; and there was a large number of fauna in the cecum of the mice. At the same time, the number of short-chain fatty acid (SCFA) bacterial producers increased after feeding with oligosaccharides; Lotus seed oligosaccharides (LOS) also enhanced the concentration of SCFAs in the intestine, which also increased the concentration of cytokines in the serum of mice. In conclusion, these findings suggest that LOS or combination with resistant starch has a better effect on relieving constipation.

Keywords: Gut microbiota; Lotus seeds; Oligosaccharides; Prebiotics; Resistant starch; Short-chain fatty acids.

MeSH terms

Animals
Body Weight / drug effects
Constipation / prevention & control*
Dose-Response Relationship, Drug
Fatty Acids, Volatile / metabolism
Feces / microbiology
Female
Gastrointestinal Microbiome / drug effects*
Gene Expression Profiling
Lotus / embryology*
Male
Mice
Mice, Inbred BALB C
Oligosaccharides / administration & dosage
Oligosaccharides / pharmacology*
Prebiotics*
Seeds / chemistry*

Substances

Fatty Acids, Volatile
Oligosaccharides
Prebiotics