Can secondary metabolites extracted from Moringa seeds suppress ammonia oxidizers to increase nitrogen use efficiency and reduce nitrate contamination in potato tubers?

Abstract

Nitrification inhibition as an alleviation tool to decrease nitrogen (N) losses and increase N use efficiency (NUE) as well as reducing NO₃^- accumulation in plants is a promising technology. No study thus far has directly or indirectly to use the secondary metabolites extracted from Moringa (Moringa oleifera Lam) seeds as nitrification inhibitors. Moringa seed extract (MSE) was studied based on its content of phenolic compounds (PC) and for its antioxidant characteristic. A 2-year field experiment and 30-day incubation experiment were conducted with three treatments of control (CK), N fertilizer (300 kg N ha^-1 and 200 mg N kg^-1 soil for the field and incubation experiment, respectively), and N fertilizer with MSE (500 ppm as a TPC) to investigate the responses of ammonia-oxidizing bacteria (AOB) and archaea (AOA) to MSE and the consequences for NUE and NO₃^- accumulation in potato tubers. Total phenolics amount was 144 mg gallic acid equivalent g^-1 MSE, while flavonoid contents were 76.6 quercetin equivalent g^-1 MSE. MSE showed antioxidant activity that was comparable to the standard antioxidants TBHQ and gallic acid. MSE application with N fertilizer retarded the nitrification process, as indicated by a higher NH₄⁺-N and lower NO₃^--N content, compared with N fertilizer application alone. NH₄⁺-N content reduced to initial CK level on Day 20 under N fertilizer application alone. However, NH₄⁺-N content decreased to initial control level on Day 30 when MSE was applied. The mechanisms resulted from curbing AOB growth by phenolic compounds (TPC, TF, TAC), leading to a delay in nitrification process. AOB increased significantly when N fertilizer was applied alone; on the contrary, AOA was not sensitive to N fertilizer (with and without MSE). Increase in NUE from 37.5% to 66.3% in potato plants under MSE application with N fertilizer was also observed compared with N fertilizer application alone. The highest NO₃^- accumulation (569 mg NO₃^- kg^-1) in tubers was recorded under N fertilizer application without MSE. MSE application significantly decreased NO₃^- accumulation (92 mg NO₃^- kg^-1) in tubers which is lower than the maximum value of accepting tubers (200 mg NO₃^- kg^-1). The highest average of N uptake, fresh and dry weight, carotenoids, chlorophyll a, chlorophyll b and nitrate reductase activity was recorded when MSE was applied with N fertilizer. Accordingly, using of Moringa extracted secondary metabolites to suppress AOB growth in the soil is a significant strategy to reduce nitrification rate and N loss from soils, and therefore increase NUE as well as reducing NO₃^- accumulation in potato tubers.

Keywords: Ammonia oxidizers; Moringa seed extract (MSE); NUE; Nitrate accumulation; Nitrogen; Potato.