Spatial Heterogeneity of Cadmium Effects on Salvia sclarea Leaves Revealed by Chlorophyll Fluorescence Imaging Analysis and Laser Ablation Inductively Coupled Plasma Mass Spectrometry

Michael Moustakas; Anetta Hanć; Anelia Dobrikova; Ilektra Sperdouli; Ioannis-Dimosthenis S Adamakis; Emilia Apostolova

doi:10.3390/ma12182953

Spatial Heterogeneity of Cadmium Effects on Salvia sclarea Leaves Revealed by Chlorophyll Fluorescence Imaging Analysis and Laser Ablation Inductively Coupled Plasma Mass Spectrometry

Materials (Basel). 2019 Sep 12;12(18):2953. doi: 10.3390/ma12182953.

Authors

Michael Moustakas¹, Anetta Hanć², Anelia Dobrikova³, Ilektra Sperdouli⁴, Ioannis-Dimosthenis S Adamakis⁵, Emilia Apostolova⁶

Affiliations

¹ Department of Botany, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece. moustak@bio.auth.gr.
² Department of Trace Element Analysis by Spectroscopy Method, Faculty of Chemistry, Adam Mickiewicz University, 61 614 Poznań, Poland. anettak@amu.edu.pl.
³ Institute of Biophysics and Biomedical Engineering, Bulgarian Academy of Science, 1113 Sofia, Bulgaria. aneli@obzor.bio21.bas.bg.
⁴ Institute of Plant Breeding and Genetic Resources, Hellenic Agricultural Organization-Demeter, Thermi, 57001 Thessaloniki, Greece. ilektras@bio.auth.gr.
⁵ Department of Botany, Faculty of Biology, National and Kapodistrian University of Athens, 157 84 Athens, Greece. iadamaki@biol.uoa.gr.
⁶ Institute of Biophysics and Biomedical Engineering, Bulgarian Academy of Science, 1113 Sofia, Bulgaria. emya@obzor.bio21.bas.bg.

Abstract

In this study, for a first time (according to our knowledge), we couple the methodologies of chlorophyll fluorescence imaging analysis (CF-IA) and laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS), in order to investigate the effects of cadmium (Cd) accumulation on photosystem II (PSII) photochemistry. We used as plant material Salvia sclarea that grew hydroponically with or without (control) 100 μM Cd for five days. The spatial heterogeneity of a decreased effective quantum yield of electron transport (Φ_PSΙΙ) that was observed after exposure to Cd was linked to the spatial pattern of high Cd accumulation. However, the high increase of non-photochemical quenching (NPQ), at the leaf part with the high Cd accumulation, resulted in the decrease of the quantum yield of non-regulated energy loss (Φ_NO) even more than that of control leaves. Thus, S. sclarea leaves exposed to 100 μM Cd exhibited lower reactive oxygen species (ROS) production as singlet oxygen (¹O₂). In addition, the increased photoprotective heat dissipation (NPQ) in the whole leaf under Cd exposure was sufficient enough to retain the same fraction of open reaction centers (q_p) with control leaves. Our results demonstrated that CF-IA and LA-ICP-MS could be successfully combined to monitor heavy metal effects and plant tolerance mechanisms.

Keywords: bioimaging; clary sage; effective quantum yield (ΦPSΙΙ); non-photochemical quenching (NPQ); photochemical quenching (qp); photoprotective mechanism; photosynthetic heterogeneity; phytoremediation; reactive oxygen species (ROS); singlet oxygen (1O2).