Green synthesis of gold nanoparticle/gelatin/protein nanogels with enhanced bioluminescence/biofluorescence

I-Hsiu Chen; Yu-Fon Chen; Jhih-Han Liou; Jinn-Tsyy Lai; Chia-Chen Hsu; Nai-Yi Wang; Jeng-Shiung Jan

doi:10.1016/j.msec.2019.110101

Green synthesis of gold nanoparticle/gelatin/protein nanogels with enhanced bioluminescence/biofluorescence

Mater Sci Eng C Mater Biol Appl. 2019 Dec:105:110101. doi: 10.1016/j.msec.2019.110101. Epub 2019 Aug 19.

Authors

I-Hsiu Chen¹, Yu-Fon Chen¹, Jhih-Han Liou¹, Jinn-Tsyy Lai², Chia-Chen Hsu², Nai-Yi Wang², Jeng-Shiung Jan³

Affiliations

¹ Department of Chemical Engineering, National Cheng Kung University, No 1, University Rd., Tainan 70101, Taiwan.
² Bioresource Collection and Research Center, Food Industry Research and Development Institute, Hsinchu 30052, Taiwan.
³ Department of Chemical Engineering, National Cheng Kung University, No 1, University Rd., Tainan 70101, Taiwan; Hierarchical Green-Energy Materials (Hi-GEM) Research Center, National Cheng Kung University, Tainan 70101, Taiwan. Electronic address: jsjan@mail.ncku.edu.tw.

PMID: 31546461
DOI: 10.1016/j.msec.2019.110101

Abstract

Here we report the green synthesis of gelatin/protein hybrid nanogels containing gold nanoparticles (AuNPs) that collectively exhibit metal-enhanced luminescence/fluorescence (MEL/MEF). The gelatin/protein nanogels, prepared by genipin cross-linking of preformed gelatin/protein polyion complexes (PICs), exhibited sizes ranging between 50 and 200 nm, depending on the weight ratio of gelatin and protein. These nanogels serve as reducing and stabilizing agents for the AuNPs, allowing for nucleation in a gel network that exhibits colloidal stability and MEL/MEF. AuNP/gelatin/HRP and AuNP/gelatin/LTF nanogels presented an ~11-fold enhancement of bioluminescence in an HRP-luminol system and a ~50-fold fluorescence enhancement when compared to free LTF in cell uptake experiments. These hybrid nanogels show promise for optically enhanced diagnosis and other therapeutic applications.

Keywords: Biosynthesis; Cross-link; Green synthesis of gold nanoparticles; Metal-enhanced fluorescence; Metal-enhanced luminescence; Nanogel; Polyion complex.

MeSH terms

Animals
Gold / chemistry*
Green Chemistry Technology*
Luminescence*
Luminescent Measurements*
Metal Nanoparticles / chemistry*
Mice
Nanogels / chemistry*
RAW 264.7 Cells

Substances

Nanogels
Gold