CYP1A1, GSTT1, IL-6 and IL-8 transcription and IL-6 secretion on umbilical endothelial cells from hypertensive pregnant women: Preliminary results

Pregnancy Hypertens. 2019 Oct:18:63-66. doi: 10.1016/j.preghy.2019.09.002. Epub 2019 Sep 18.

Abstract

The impact of pregnancy hypertension in the offspring endothelia remains unknown. We evaluated the transcriptional expression of four genes that participate in the process of endothelial dysfunction using umbilical vein endothelial cell cultures (HUVEC) from healthy pregnant women (PW) and those with hypertensive disorders (HD). The cytochrome P450 1A1 (CYP1A1), gluthathione S-transferase subtype T1 (GSTT1), interleukin 6 (IL-6) and 8 (IL-8) mRNA and IL-6 protein levels were assessed. IL-6 and IL-8 transcripts were significantly reduced in HUVEC obtained from HD women. Our results suggest that a hypertensive environment in utero modifies the transcriptional expression of key inflammatory molecules in the newborn.

Keywords: CYP1A1; Endothelial cells; Glutathione S-transferase T1; Interleukin-6; Interleukin-8; Pregnancy hypertension.

MeSH terms

  • Adult
  • Case-Control Studies
  • Cytochrome P-450 CYP1A1 / genetics
  • Cytochrome P-450 CYP1A1 / metabolism
  • Endothelium, Vascular / physiopathology*
  • Female
  • Glutathione Transferase / genetics
  • Glutathione Transferase / metabolism
  • Human Umbilical Vein Endothelial Cells
  • Humans
  • Hypertension / metabolism*
  • Interleukin-6 / genetics
  • Interleukin-6 / metabolism
  • Interleukin-8 / metabolism
  • Pre-Eclampsia / metabolism
  • Pregnancy
  • Pregnancy Complications, Cardiovascular / metabolism*
  • Transcription, Genetic
  • Umbilical Veins / cytology
  • Young Adult

Substances

  • Interleukin-6
  • Interleukin-8
  • CYP1A1 protein, human
  • Cytochrome P-450 CYP1A1
  • glutathione S-transferase T1
  • Glutathione Transferase