Iron Fortification and Bioavailability of Chickpea (Cicer arietinum L.) Seeds and Flour

Nutrients. 2019 Sep 18;11(9):2240. doi: 10.3390/nu11092240.

Abstract

Iron (Fe) deficiency is one of the most common nutritional disorders, and is mainly due to insufficient intake of bioavailable Fe. Chickpea (Cicer arietinum L.) was examined as a potential vehicle for Fe fortification. Fortificants (FeSO4·7H2O (ferrous sulfate hepta-hydrate), FeSO4·H2O (ferrous sulfate mono-hydrate) and NaFeEDTA (ethylenediaminetetraacetic acid iron (iii) sodium salt)) were applied by a spraying and drying method. At 2000 µg g-1 iron fortificant, the fortified split desi seeds (dal), desi flour and kabuli flour supplied 18-19 mg, 16-20 mg and 11-19 mg Fe per 100 g, respectively. The overall consumer acceptability using a nine-point hedonic scale for sensory evaluation demonstrated that NaFeEDTA-fortified cooked chickpea (soup and chapatti) scored the highest among the three fortificants. Lightness (L*), redness (a*) and yellowness (b*) of Fe-fortified products changed over time. However, no organoleptic changes occurred. Fe bioavailability was increased by 5.8-10.5, 15.3-25.0 and 4.8-9.0 ng ferritin mg-1 protein for cooked split desi seeds (soup), desi chapatti and kabuli chapatti, respectively, when prepared using Fe-fortified chickpea. Desi chapatti showed significantly higher Fe bioavailability than the other two. The increase in Fe concentration and bioavailability in fortified chickpea products demonstrated that these products could provide a significant proportion of the recommended daily Fe requirement.

Keywords: NaFeEDTA; bioavailability; chickpea; fortification; hedonic scale; iron.

MeSH terms

  • Adult
  • Biological Availability
  • Cicer / chemistry*
  • Cooking
  • Edetic Acid
  • Ferric Compounds
  • Ferrous Compounds
  • Flour / analysis*
  • Food Preferences
  • Food, Fortified / analysis*
  • Humans
  • Iron* / analysis
  • Iron* / pharmacokinetics
  • Middle Aged
  • Seeds / chemistry*
  • Young Adult

Substances

  • Ferric Compounds
  • Ferrous Compounds
  • ferrous sulfate
  • Edetic Acid
  • Iron
  • Fe(III)-EDTA