Atomic force microscopy-based single-molecule force spectroscopy detects DNA base mismatches

Wenjing Liu; Yourong Guo; Kaizhe Wang; Xingfei Zhou; Ying Wang; Junhong Lü; Zhifeng Shao; Jun Hu; Daniel M Czajkowsky; Bin Li

doi:10.1039/c9nr05234h

Atomic force microscopy-based single-molecule force spectroscopy detects DNA base mismatches

Nanoscale. 2019 Oct 7;11(37):17206-17210. doi: 10.1039/c9nr05234h. Epub 2019 Sep 17.

Authors

Wenjing Liu¹, Yourong Guo², Kaizhe Wang¹, Xingfei Zhou³, Ying Wang⁴, Junhong Lü⁴, Zhifeng Shao², Jun Hu⁵, Daniel M Czajkowsky², Bin Li⁴

Affiliations

¹ Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China. hujun@sinap.ac.cn libin@sinap.ac.cn and University of Chinese Academy of Sciences, Beijing 100049, China.
² Bio-ID Center, School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, 200240, China. dczaj@sjtu.edu.cn.
³ School of Science, Ningbo University, Ningbo 315211, Zhejiang, China.
⁴ Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China. hujun@sinap.ac.cn libin@sinap.ac.cn and Zhangjiang Laboratory, Shanghai Advanced Research Institute, Chinese Academy of Sciences, Shanghai 201210, China.
⁵ Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China. hujun@sinap.ac.cn libin@sinap.ac.cn and Zhangjiang Laboratory, Shanghai Advanced Research Institute, Chinese Academy of Sciences, Shanghai 201210, China and School of Physical Science and Technology, Shanghai Tech University, Shanghai 201204, China.

PMID: 31535117
DOI: 10.1039/c9nr05234h

Abstract

Atomic force microscopy-based single-molecule-force spectroscopy is limited by low throughput. We introduce addressable DNA origami to study multiple target molecules. Six target DNAs that differed by only a single base-pair mismatch were clearly differentiated a rupture force of only 4 pN.

MeSH terms

Base Pair Mismatch*
DNA / chemistry*
DNA / genetics*
Microscopy, Atomic Force*

Substances

DNA