Multilocus Sequence Typing of Staphylococcus aureus

Ying Liu; Yinduo Ji

doi:10.1007/978-1-4939-9849-4_7

Multilocus Sequence Typing of Staphylococcus aureus

Methods Mol Biol. 2020:2069:95-102. doi: 10.1007/978-1-4939-9849-4_7.

Authors

Ying Liu¹, Yinduo Ji²

Affiliations

¹ Shanghai Vocational College of Agriculture and Forestry, Shanghai, China.
² Department of Veterinary Biomedical Sciences, College of Veterinary Medicine, University of Minnesota, Saint Paul, MN, USA. jixxx002@umn.edu.

PMID: 31523768
DOI: 10.1007/978-1-4939-9849-4_7

Abstract

Multilocus sequence typing (MLST) has been successfully used to differentiate and trace the bacterial species and pathogens that cause outbreaks or epidemics of infectious diseases. MLST provides a powerful solution for molecular epidemiological characterization of bacterial strains, including Staphylococcus aureus, by using the sequences of the internal region of seven housekeeping genes. In previous studies, we utilized MLST to analyze the genotypes of S. aureus isolates from pediatric patients with cystic fibrosis and revealed three prevalent ST types, including ST5, ST30, and ST8 in these isolates. In this chapter, we describe a detailed procedure of MLST for genotyping S. aureus.

Keywords: Genotyping; MLST; MRSA; Staphylococcus aureus.

MeSH terms

Bacterial Typing Techniques*
Genes, Bacterial*
Genotype*
Genotyping Techniques*
Humans
Multilocus Sequence Typing*
Staphylococcus aureus* / classification
Staphylococcus aureus* / genetics