Simulated digestion and fermentation in vitro by human gut microbiota of polysaccharides from Helicteres angustifolia L

Ligen Chen; Junwei Liu; Xiaodong Ge; Wei Xu; Yun Chen; Fengwei Li; Delin Cheng; Rong Shao

doi:10.1016/j.ijbiomac.2019.09.073

Simulated digestion and fermentation in vitro by human gut microbiota of polysaccharides from Helicteres angustifolia L

Int J Biol Macromol. 2019 Dec 1:141:1065-1071. doi: 10.1016/j.ijbiomac.2019.09.073. Epub 2019 Sep 10.

Authors

Ligen Chen¹, Junwei Liu², Xiaodong Ge³, Wei Xu⁴, Yun Chen⁵, Fengwei Li⁵, Delin Cheng⁶, Rong Shao²

Affiliations

¹ Department of Bioengineering, School of Marine and Bioengineering, Yancheng Institute of Technology, Yancheng 224051, China; Jiangsu Key Laboratory of Biochemistry and Biotechnology of Marine Wetland, Yancheng Institute of Technology, Yancheng 224051, China. Electronic address: 611081704003@njtech.edu.cn.
² Department of Bioengineering, School of Marine and Bioengineering, Yancheng Institute of Technology, Yancheng 224051, China; Jiangsu Key Laboratory of Biochemistry and Biotechnology of Marine Wetland, Yancheng Institute of Technology, Yancheng 224051, China.
³ College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
⁴ Department of Bioengineering, School of Marine and Bioengineering, Yancheng Institute of Technology, Yancheng 224051, China; Jiangsu Key Laboratory of Biochemistry and Biotechnology of Marine Wetland, Yancheng Institute of Technology, Yancheng 224051, China. Electronic address: ycitxuwei@ycit.edu.cn.
⁵ Department of Bioengineering, School of Marine and Bioengineering, Yancheng Institute of Technology, Yancheng 224051, China.
⁶ Yancheng Kanglinda Biotechnology Co.,Ltd., Yancheng 224051, China.

PMID: 31518623
DOI: 10.1016/j.ijbiomac.2019.09.073

Abstract

Helicteres angustifolia L. is a familiar herbal plant, which exhibits various bioactivities with potential benefits for human health. In this study, the simulated digestion (saliva, simulated gastric and small intestinal conditions) and fermentation in vitro of polysaccharides from H. angustifolia L. (HaLPs) were evaluated, and the effects of HaLPs on gut microbiota were examined using high-throughput sequencing technology. The results indicated that the simulated gastrointestinal digestion had no effect on HaLPs, so HaLPs could reach the large intestine safely. However, the molecular weight of HaLPs and the reducing sugar decreased significantly after fermentation under anaerobic conditions. It was found that HaLPs had the significantly alternation effect on the composition of the gut microbiota. Furthermore, the total short-chain fatty acids content increased significantly after fermentation (from 2.25 ± 0.13 mM to 22.45 ± 4.56 mM). These results provided an understanding of the digestive characteristics of HaLPs and afforded a viable basis for their development.

Keywords: Digestion; Gut microbiota; Helicteres angustifolia L.; Polysaccharides.

MeSH terms

Biomimetics*
Digestion*
Fermentation*
Gastrointestinal Microbiome* / drug effects
Humans
Malvaceae / chemistry*
Polysaccharides / metabolism*
Polysaccharides / pharmacology

Substances

Polysaccharides