Expression, purification and crystallization of an SLC16 monocarboxylate transporter family homologue specific for l-lactate

Protein Expr Purif. 2020 Jan:165:105484. doi: 10.1016/j.pep.2019.105484. Epub 2019 Sep 9.

Abstract

l-lactate plays an important role as metabolite and signaling molecule in eukaryotes and bacteria. Monocarboxylate transporters (MCTs) of the SLC16 solute carrier family are responsible for the transport of l-lactate across eukaryotic and bacterial cell membranes. Here we report an efficient protocol for the expression and purification of an SLC16 family homologue in milligram amounts. The purified protein is stable and can thus be used for biochemical and structural studies as shown by successful crystallization.

Keywords: Expression of membrane proteins; Membrane protein; Monocarboxylate transporter; Purification of membrane proteins; SLC16 family; Structure; l-lactate transporter.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Cell Membrane / metabolism
  • Cell Membrane Permeability
  • Chromatography, Gel
  • Cloning, Molecular
  • Crystallization*
  • Deltaproteobacteria / genetics*
  • Escherichia coli / genetics
  • Gene Expression
  • Lactates / metabolism*
  • Monocarboxylic Acid Transporters / genetics*
  • Monocarboxylic Acid Transporters / isolation & purification*
  • Protein Stability
  • Transfection

Substances

  • Lactates
  • Monocarboxylic Acid Transporters

Supplementary concepts

  • Syntrophobacter fumaroxidans