Recent evidences suggested that miRNAs process key roles in the biological behaviors and the development of prostate carcinoma (PC). However, its molecular mechanism in PC is still remained largely unclear. In this study, the expression level of miR-188 and the protein expression of MARCKS were detected by RT-PCR and western blot, respectively. Moreover, we determined the oncogene or anti-oncogene role of miR-188 in the PC through gain and loss function assay of miR-188 using transfection of miR-188 mimics and inhibitor. Meanwhile, luciferase reporter vectors with miR-188 mimics were constructed and transfected into PC cells. Our data indicated that the expression of miR-188 was dramatically reduced in human PC tissues and cell lines and the increased miR-188 levels obviously inhibited the proliferation, migration and invasion of PC cells and negatively regulative with MARCKS. Together, our findings proved that miR-188 plays an inhibitive role in PC of proliferation, migration and invasion at least in part via suppressing MARCKS expression, which could be regarded as a promising therapeutic target in PC.
Keywords: MARCKS; invasion; miR-188; proliferation; prostate carcinoma.