A two-stage modification method using 1,4-α-glucan branching enzyme lowers the in vitro digestibility of corn starch

Junyan Ren; Shuangdi Chen; Caiming Li; Zhengbiao Gu; Li Cheng; Yan Hong; Zhaofeng Li

doi:10.1016/j.foodchem.2019.125441

A two-stage modification method using 1,4-α-glucan branching enzyme lowers the in vitro digestibility of corn starch

Food Chem. 2020 Feb 1:305:125441. doi: 10.1016/j.foodchem.2019.125441. Epub 2019 Aug 29.

Authors

Junyan Ren¹, Shuangdi Chen¹, Caiming Li², Zhengbiao Gu³, Li Cheng², Yan Hong⁴, Zhaofeng Li⁵

Affiliations

¹ School of Food Science and Technology, Jiangnan University, Wuxi 214122, China.
² State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China; School of Food Science and Technology, Jiangnan University, Wuxi 214122, China; Collaborative innovation center of food safety and quality control in Jiangsu Province, Jiangnan University, Wuxi, Jiangsu 214122, China.
³ State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China; School of Food Science and Technology, Jiangnan University, Wuxi 214122, China; Collaborative innovation center of food safety and quality control in Jiangsu Province, Jiangnan University, Wuxi, Jiangsu 214122, China. Electronic address: zhengbiaogu@jiangnan.edu.cn.
⁴ State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China; School of Food Science and Technology, Jiangnan University, Wuxi 214122, China; Collaborative innovation center of food safety and quality control in Jiangsu Province, Jiangnan University, Wuxi, Jiangsu 214122, China. Electronic address: hongyan@jiangnan.edu.cn.
⁵ State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China; School of Food Science and Technology, Jiangnan University, Wuxi 214122, China; Collaborative innovation center of food safety and quality control in Jiangsu Province, Jiangnan University, Wuxi, Jiangsu 214122, China. Electronic address: zfli@jiangnan.edu.cn.

PMID: 31494499
DOI: 10.1016/j.foodchem.2019.125441

Abstract

Samples of granular corn starch were treated with 1,4-α-glucan branching enzyme (GBE) for 20 h using three different methods. These GBE modification methods all increased glycosidic linkage ratio, cyclic glucan content, and proportion of short chains while reducing weight mean molecular weight. The in vitro digestion rates of the modified starches were suppressed. Among these methods, a novel two-stage modification method comprising a 10-h GBE treatment, gelatinization, and a second 10-h GBE treatment, produced samples with the lowest in vitro digestibility. The rapidly digestible starch content was 34.2% lower than that of the control and 18.0% lower than that of the product of one-stage modification with the same duration. Fine structure characterization showed that more cluster structures were proved during the two-stage modification. This two-stage method suppressed the digestibility of corn starch and increased the substrate concentration, showing great potential for the industrial processing of slowly-digestible starchy foods.

Keywords: 1,4-α-glucan branching enzyme; Cluster structure; Digestion rate; Functional foods; Glycemic index.

MeSH terms

1,4-alpha-Glucan Branching Enzyme / metabolism*
Glucans / analysis
Magnetic Resonance Spectroscopy
Molecular Weight
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Starch / metabolism*
Zea mays / metabolism*

Substances

Glucans
Starch
1,4-alpha-Glucan Branching Enzyme